Department of Rheumatology, The Southern Medical University, Guangzhou.
Dis Esophagus. 2013 Nov-Dec;26(8):817-22. doi: 10.1111/j.1442-2050.2012.01400.x. Epub 2012 Sep 28.
The ataxia-telangiectasia group D complementing gene (ATDC) plays significant roles in various human cancers. However, the clinical significance of ATDC in esophageal squamous cell carcinoma (ESCC) has not been investigated. The ATDC messenger RNA level of 40 paired ESCC and nonneoplastic tissues were evaluated using quantitative real-time polymerase chain reaction, 10 pairs of which were also used for Western blot analysis. In addition, immunohistochemical staining was performed to detect the ATDC expression in 118 paraffin-embedded cancerous and matched nonneoplastic tissues, and the correlation of ATDC expression with the clinicopathological parameters and prognosis of the ESCC patients was analyzed. The quantitative real-time polymerase chain reaction, immunohistochemical staining, and Western blot results demonstrated that the expression level of ATDC was significantly higher in ESCC tissue than in matched noncancerous tissues. Both ATDC messenger RNA and protein expression in the ESCC tissue were significantly correlated with tumor differentiation, stage, and lymph node metastasis. However, there was no significant difference in ATDC expression based on patient age or gender. Moreover, the results of both univariate and multivariate analyses showed that increased ATDC expression was correlated with a shorter 5-year survival time for ESCC patients after surgery. We concluded that increased ATDC expression is associated with poor clinical outcomes and that this marker might be a useful indicator for prognosis and a promising target for therapy in ESCC patients.
共济失调毛细血管扩张症突变基因 D 互补基因（ATDC）在多种人类癌症中发挥着重要作用。然而，ATDC 在食管鳞状细胞癌（ESCC）中的临床意义尚未被研究。采用实时定量聚合酶链反应（PCR）检测 40 对 ESCC 及非肿瘤组织中 ATDC 的信使 RNA 水平，其中 10 对也用于 Western blot 分析。此外，采用免疫组织化学染色检测 118 例石蜡包埋的癌组织及匹配的非肿瘤组织中 ATDC 的表达情况，并分析 ATDC 表达与 ESCC 患者临床病理参数及预后的相关性。实时定量 PCR、免疫组织化学染色和 Western blot 结果表明，ATDC 在 ESCC 组织中的表达水平明显高于匹配的非癌组织。ESCC 组织中 ATDC 的信使 RNA 和蛋白表达均与肿瘤分化、分期和淋巴结转移显著相关。然而，ATDC 的表达与患者年龄或性别无关。此外，单因素和多因素分析的结果均表明，术后 ATDC 表达增加与 ESCC 患者 5 年生存率缩短相关。我们得出结论，ATDC 表达增加与不良临床结局相关，该标志物可能是 ESCC 患者预后的有用指标和有前途的治疗靶点。
