Zhu Xiaolei, Jin Huan, Xia Ziwei, Wu Xianxian, Yang Mingjian, Zhang Hongdian, Shang Xiaobin, Cheng Runfen, Zhan Zhongli, Yu Zhentao
Department of Esophageal Cancer, Tianjin Medical University Cancer Institute and Hospital, Key Laboratory of Cancer Prevention and Therapy of Tianjin City, Tianjin 300060, China; Department of Thoracic Surgery, Second Hospital of Tianjin Medical University, Tianjin 300060, China.
Department of Nursing, Second Hospital of Tianjin Medical University, Tianjin 300060, China.
Biochem Biophys Res Commun. 2017 Apr 29;486(2):571-576. doi: 10.1016/j.bbrc.2017.03.091. Epub 2017 Mar 20.
Recently, Vav1 has been suggested to play an essential role in the progression of human cancers. However, the correlation between Vav1 expression and prognosis of esophageal squamous cell carcinoma (ESCC) is still unknown. The aim of this study was to investigate Vav1 expression and its prognostic value in ESCC. The expression of Vav1 was detected by real-time quantitative reverse transcription polymerase chain reaction (qRT-PCR) and Western blotting in ESCC tissues and matched nontumorous tissues. Immunohistochemistry (IHC) was carried out to detect Vav1 expression in paraffin samples from 112 primary ESCC patients. Survival analysis was performed using Kaplan-Meier method. Univariate and multivariate Cox regression analyses were performed to evaluate the correlation of Vav1 expression with prognosis of ESCC patients. The expression levels of Vav1 mRNA and protein in ESCC tissues were both significantly higher than those in adjacent nontumorous tissues. High Vav1 expression was significantly correlated with larger tumor size (P = 0.015), depth of tumor invasion (P = 0.023), lymph node metastasis (P = 0.008) and TNM stage (P < 0.001). The rate of overall survival (OS) was significantly lower in patients with high Vav1 expression than those with low Vav1 expression (P = 0.014). Multivariate Cox analysis indicated that Vav1 expression is an independent prognostic factor for OS (HR = 1.660, 95%CI = 1.058-2.607, P = 0.028). In summary, our findings demonstrate that Vav1 may be a candidate molecular prognostic marker for patients with ESCC.
最近，有人提出Vav1在人类癌症进展中起关键作用。然而，Vav1表达与食管鳞状细胞癌（ESCC）预后之间的相关性仍不清楚。本研究的目的是探讨Vav1在ESCC中的表达及其预后价值。通过实时定量逆转录聚合酶链反应（qRT-PCR）和蛋白质免疫印迹法检测ESCC组织及配对的非肿瘤组织中Vav1的表达。采用免疫组织化学（IHC）检测112例原发性ESCC患者石蜡样本中Vav1的表达。采用Kaplan-Meier法进行生存分析。进行单因素和多因素Cox回归分析，以评估Vav1表达与ESCC患者预后的相关性。ESCC组织中Vav1 mRNA和蛋白的表达水平均显著高于相邻的非肿瘤组织。Vav1高表达与肿瘤较大尺寸（P = 0.015）、肿瘤浸润深度（P = 0.023）、淋巴结转移（P = 0.008）和TNM分期（P < 0.001）显著相关。Vav1高表达患者的总生存率（OS）显著低于Vav1低表达患者（P = 0.014）。多因素Cox分析表明，Vav1表达是OS的独立预后因素（HR = 1.660，95%CI = 1.058 - 2.607，P = 0.028）。总之，我们的研究结果表明，Vav1可能是ESCC患者的候选分子预后标志物。
