人内源性逆转录病毒长末端重复序列（LTR）在肺癌细胞系中的转录

Transcription of human endogenous retroviral long terminal repeat (LTR) sequence in a lung cancer cell line.

作者信息

Tomita N, Horii A, Doi S, Yokouchi H, Ogawa M, Mori T, Matsubara K

机构信息

Institute for Molecular and Cellular Biology, Osaka University, Suita, Japan.

出版信息

Biochem Biophys Res Commun. 1990 Jan 15;166(1):1-10. doi: 10.1016/0006-291x(90)91904-7.

DOI:10.1016/0006-291x(90)91904-7

PMID:2302192

Abstract

The human genome carries several endogenous retroviral sequences. One of them that we named 'HERV-A', carries almost the complete sequence of the long terminal repeat (LTR), and is located in the 5' region of the amylase genes (M.Emi, A.Horii, N.Tomita, T.Nishide, M.Ogawa, T. Mori and K.Matsubara, Gene 62: 229-235, 1988). Using this sequence as a probe, we found a 1.4 kb LTR transcript(s) in a lung cancer cell line. No corresponding transcript was observed in control cells. Two partial, but different cDNA clones were obtained, and each one was found to be a transcript starting within human sequences at 5' upstream from the LTR and ending within the LTR sequence.

摘要

人类基因组携带多个内源性逆转录病毒序列。其中一个我们命名为“HERV - A”的序列，几乎携带了长末端重复序列（LTR）的完整序列，并且位于淀粉酶基因的5'区域（M. Emi、A. Horii、N. Tomita、T. Nishide、M. Ogawa、T. Mori和K. Matsubara，《基因》62: 229 - 235，1988年）。以该序列为探针，我们在一种肺癌细胞系中发现了一种1.4 kb的LTR转录本。在对照细胞中未观察到相应的转录本。获得了两个部分但不同的cDNA克隆，并且发现每个克隆都是一个转录本，其起始于LTR 5'上游的人类序列内，终止于LTR序列内。