Wiland E, Siemieniako B, Trzeciak W H
Institute of Human Genetics, Polish Academy of Sciences, Poznań, Poland.
Biochem Biophys Res Commun. 1990 Jan 15;166(1):11-21. doi: 10.1016/0006-291x(90)91905-8.
The protein of molecular weight about 160 kD (designated LMG160) was isolated from purified low mobility group chromatin proteins. Polyclonal antibody directed against the LMG160 protein in mouse was raised. The specificity of the antibody was determined with the use of ELISA. Using chemical cross-linking procedure followed by immunoprecipitation with the antiLMG160 antibody complex formation with chromatin proteins was demonstrated. Among the proteins that form complexes with LMG160, histones H3, H2A, and H4 were identified (Western blotting technique).
从纯化的低迁移率组蛋白中分离出分子量约为160 kD的蛋白质（命名为LMG160）。制备了针对小鼠LMG160蛋白的多克隆抗体。使用酶联免疫吸附测定法（ELISA）确定抗体的特异性。通过化学交联程序，随后用抗LMG160抗体进行免疫沉淀，证明了与染色质蛋白形成复合物。在用LMG160形成复合物的蛋白质中，鉴定出了组蛋白H3、H2A和H4（蛋白质免疫印迹技术）。
