Espel E, Bernués J, Pérez-Pons J A, Querol E
Biochem Biophys Res Commun. 1985 Nov 15;132(3):1031-7. doi: 10.1016/0006-291x(85)91910-2.
The interaction between calf thymus HMG14 and rat liver chromatin components has been studied via reconstitution and chemical cross-linking. Selective labeling of HMG14 with photoactivable reversible heterobifunctional reagents has allowed a clear identification of the histones interacting with it (histones H2A, H2B and H1). These results are not dependent on whether the chromatin samples used were bulk chromatin, mononucleosomes, or core particles (for H2A and H2B). In addition to histone proteins, DNA also seems to be involved in HMG14 attachment to nucleosome.
通过重组和化学交联研究了小牛胸腺HMG14与大鼠肝脏染色质成分之间的相互作用。用可光活化的可逆异双功能试剂对HMG14进行选择性标记，从而能够明确鉴定与其相互作用的组蛋白（组蛋白H2A、H2B和H1）。这些结果不取决于所使用的染色质样品是整体染色质、单核小体还是核心颗粒（对于H2A和H2B）。除组蛋白外，DNA似乎也参与了HMG14与核小体的结合。
