缓激肽对神经培养物中单酰甘油脂肪酶和二酰甘油脂肪酶活性的刺激作用。

Stimulation of mono- and diacylglycerol lipase activities by bradykinin in neural cultures.

作者信息

Farooqui A A, Anderson D K, Flynn C, Bradel E, Means E D, Horrocks L A

机构信息

Department of Physiological Chemistry, Ohio State University, Columbus 43210.

出版信息

Biochem Biophys Res Commun. 1990 Jan 30;166(2):1001-9. doi: 10.1016/0006-291x(90)90910-f.

DOI:10.1016/0006-291x(90)90910-f

PMID:2302218

Abstract

Neural cultures of fetal mouse spinal cord, mouse neuroblastoma (N1E-115) and mixed primary glial cell cultures from neonatal rat brain display measurable activities of mono- and diacylglycerol lipases. Treatment of fetal mouse spinal cord cultures with bradykinin (10 nM) for 1-4 min resulted in a marked increase in specific activities of mono- and diacylglycerol lipases. This is the first direct demonstration that bradykinin can act through the lipase pathway. The increase in activities of lipases was dose and time dependent. The bradykinin response was blocked by [Thi5,8, D-Phe7]bradykinin, a bradykinin B-2 receptor antagonist, indicating that the bradykinin induced stimulation of lipase activities involves bradykinin receptors.

摘要

胎鼠脊髓的神经培养物、小鼠神经母细胞瘤（N1E-115）以及新生大鼠脑的混合原代神经胶质细胞培养物均显示出单酰甘油脂肪酶和二酰甘油脂肪酶的可测量活性。用缓激肽（10 nM）处理胎鼠脊髓培养物1至4分钟，导致单酰甘油脂肪酶和二酰甘油脂肪酶的比活性显著增加。这是缓激肽可通过脂肪酶途径起作用的首次直接证明。脂肪酶活性的增加呈剂量和时间依赖性。缓激肽反应被缓激肽B-2受体拮抗剂[Thi5,8, D-Phe7]缓激肽阻断，表明缓激肽诱导的脂肪酶活性刺激涉及缓激肽受体。