大鼠心脏中的脂蛋白脂肪酶——I. 肝素后流出物中三酰甘油、二酰甘油和单酰甘油脂肪酶活性的表征

Lipoprotein lipase in rat heart--I. Characterization of tri-, di- and monoacylglycerol lipase activities in post-heparin effluents.

Benkirane M, Meignen J M, Boyer J, Verine A

INSERM U.260, Faculté de Médecine, Marseille, France.

Comp Biochem Physiol B. 1989;94(1):13-8. doi: 10.1016/0305-0491(89)90003-5.

Abstract

The lipolytic activities that sequentially hydrolyze tri-, di- and monoacylglycerol in rat post-heparin heart effluents were examined. 2. Properties of triacylglycerol lipase (TAGL) activity were typical of lipoprotein lipase. Diacylglycerol lipase (DAGL) behaved similarly to TAGL, suggesting that both activities refer to the same catalytic entity. 3. Differences, particularly in thermal stability, between TAGL and DAGL activities on one hand, and monoacylglycerol lipase (MAGL) activity on the other, may reflect different intrinsic molecular properties. 4. TAGL, DAGL and MAGL activities could not be separated by physical means and appeared to belong to a single unit at the same site on the capillary wall.

摘要

对大鼠肝素化心脏流出液中依次水解三酰甘油、二酰甘油和单酰甘油的脂解活性进行了检测。2. 三酰甘油脂肪酶（TAGL）活性的特性是脂蛋白脂肪酶的典型特性。二酰甘油脂肪酶（DAGL）的行为与TAGL相似，这表明这两种活性指的是同一催化实体。3. 一方面，TAGL和DAGL活性与另一方面的单酰甘油脂肪酶（MAGL）活性之间的差异，尤其是热稳定性方面的差异，可能反映了不同的内在分子特性。4. TAGL、DAGL和MAGL活性无法通过物理方法分离，并且似乎在毛细血管壁的同一位置属于单个单元。