State Key Lab of Virology, College of Life Sciences, Wuhan University, Wuhan 430072, China.
State Key Lab of Virology, College of Life Sciences, Wuhan University, Wuhan 430072, China.
Vet Microbiol. 2013 Mar 23;162(2-4):321-329. doi: 10.1016/j.vetmic.2012.09.007. Epub 2012 Sep 12.
With the aid of ammonium chloride, we established a model for persistent foot-and-mouth disease virus (FMDV) infection of BHK-21 cells (Huang et al., 2011). Distinctive to a previously established model, the persistently infected cell line acquired new features including more rounded morphology, resistance to wild type FMDV infection, consistent replication efficiency in late passages, etc. To elucidate the mechanism of establishment of persistence, we performed systematically microarray analysis of gene expression profiles of acute and persistent infections and real-time quantitative PCR validation of key genes. Our results showed 12 common genes were found to be up-regulated in acute infection while down-regulated in persistent infection. Gene expression analysis indicated differences in the KEGG pathway, revealing important roles of host factors in the maintenance of symbiotic environment. The results suggest that, in contrast to previous viral persistence system, the critical element in establishment of the persistence in our lab is the evolution of host cells which regulate genome transcription to defy the lytic effects of FMDV infection.
在氯化铵的帮助下，我们建立了一种持续感染口蹄疫病毒（FMDV）的 BHK-21 细胞模型（Huang 等人，2011 年）。与之前建立的模型不同，持续感染的细胞系获得了新的特征，包括更圆的形态、对野生型 FMDV 感染的抗性、在后期传代中的稳定复制效率等。为了阐明建立持续性的机制，我们对急性和持续性感染的基因表达谱进行了系统的微阵列分析，并对关键基因进行了实时定量 PCR 验证。我们的结果表明，在急性感染中发现 12 个共同上调的基因，而在持续性感染中下调。基因表达分析表明，KEGG 途径存在差异，表明宿主因子在维持共生环境中发挥着重要作用。结果表明，与以前的病毒持续性系统不同，本实验室建立持续性的关键因素是宿主细胞的进化，它调节基因组转录以抵抗 FMDV 感染的裂解效应。
