Referral Center for Pediatric Gastroenterology and Nutrition, Children's Hospital Zagreb, School of Medicine, University of Zagreb, Zagreb, Croatia.
Eur J Gastroenterol Hepatol. 2013 Jan;25(1):22-7. doi: 10.1097/MEG.0b013e328359526c.
The aims of this research were to determine the number of chromosomal aberrations in peripheral blood lymphocytes and to evaluate the number of circulating lymphocytes with CD103, integrin expressed on intraepithelial lymphocytes and preserved in enteropathy-associated T-cell lymphoma, in patients with newly diagnosed Crohn's disease, celiac disease, and healthy controls.
During the period of 30 months, we included 44 patients. Chromosome aberrations were analyzed in peripheral blood lymphocytes by a single cytogeneticist. Multicolor flow cytometric was used for immunophenotyping of peripheral blood lymphocytes.
We found a significantly higher number of chromosomal aberrations/100 metaphases in the celiac and Crohn's disease group compared with the controls (P=0.01) and they also had a significantly higher number of aberrant cells compared with the controls (P<0.001). There was no statistically significant difference between the groups with respect to the percentage of CD103+ and CD8+CD103+ cells between groups (P=0.16 and 0.41, respectively) and no correlation between the total number of chromosomal aberrations and the percentage of CD103+ and CD8+CD103+ cells (P=0.06 and 0.06, respectively).
Patients with active celiac and newly diagnosed Crohn's disease, before treatment initiation, have a significantly increased number of chromosomal aberrations in peripheral blood lymphocytes. No dissemination of intraepithelial cells in the blood and correlation to the chromosomal aberration was found.
本研究旨在确定外周血淋巴细胞中染色体畸变的数量，并评估在新诊断的克罗恩病、乳糜泻和健康对照者中，循环淋巴细胞中表达上皮内淋巴细胞上的 CD103 和整合素的数量。
在 30 个月的时间内，我们纳入了 44 名患者。由一名细胞遗传学家分析外周血淋巴细胞中的染色体畸变。采用多色流式细胞术对外周血淋巴细胞进行免疫表型分析。
我们发现乳糜泻和克罗恩病组的染色体畸变/100 个中期明显高于对照组（P=0.01），且与对照组相比，异常细胞数量也明显更高（P<0.001）。在 CD103+和 CD8+CD103+细胞的百分比方面，各组之间没有统计学上的显著差异（分别为 P=0.16 和 0.41），并且总染色体畸变数与 CD103+和 CD8+CD103+细胞的百分比之间没有相关性（分别为 P=0.06 和 0.06）。
在开始治疗之前，患有活动性乳糜泻和新诊断的克罗恩病的患者外周血淋巴细胞中的染色体畸变数量明显增加。未发现上皮内细胞在血液中的扩散与染色体畸变有关。
