慢性中枢注射脂多糖在转录水平上上调大鼠海马中连接蛋白30和32间隙连接。
Upregulation of connexins 30 and 32 gap junctions in rat hippocampus at transcription level by chronic central injection of lipopolysaccharide.
作者信息
Abbasian Mohammad, Sayyah Mohammad, Babapour Vahab, Mahdian Reza, Choopani Samira, Kaviani Bahar
机构信息
Dept. of Physiology and Pharmacology, Pasteur Institute of Iran, Tehran, Iran.
Dept. of Physiology and Pharmacology, Pasteur Institute of Iran, Tehran; 3Dept. of Physiology, Faculty of Veterinary Medicine, Tehran University, Tehran, Iran.
出版信息
Iran Biomed J. 2012;16(3):127-32. doi: 10.6091/ibj.1099.2012.
BACKGROUND
Gap junctions composed of connexins (Cx) are functional in cell defense by propagation of toxic/death molecules to neighboring cells. Hippocampus, one of the brain regions with particular vulnerability to damage, has a wide network of gap junctions. Functional response of astrocytic Cx30 and neuronal Cx32 to hippocampal damage is unknown.
METHODS
We infused lipopolysaccharide (LPS) intracerebroventricularly (2.5 mug/rat) once daily for two weeks to create neuroinflammation. The mRNA and protein levels of the Cx were measured in the hippocampus after 1st, 7th and 14th injection by real-time PCR and Western-blot techniques.
RESULTS
A significant increase in Cx32 and Cx30 gene expression was observed after 7th and 14th injection of LPS with no significant change in their protein abundance.
CONCLUSION
Transcriptional overexpression of hippocampal Cx30 and Cx32 could be an adaptive response to production of intracellular toxic molecules but it is not accompanied with post- transcriptional overexpression and might have no functional impact.
背景
由连接蛋白（Cx）组成的间隙连接通过将毒性/死亡分子传播到邻近细胞在细胞防御中发挥作用。海马体是特别易受损伤的脑区之一，具有广泛的间隙连接网络。星形胶质细胞Cx30和神经元Cx32对海马体损伤的功能反应尚不清楚。
方法
我们每天一次脑室内注射脂多糖（LPS，2.5微克/只大鼠），持续两周以引发神经炎症。在第1次、第7次和第14次注射后，通过实时PCR和蛋白质印迹技术测量海马体中Cx的mRNA和蛋白质水平。
结果
在第7次和第14次注射LPS后，观察到Cx32和Cx30基因表达显著增加，但其蛋白质丰度无显著变化。
结论
海马体Cx30和Cx32的转录过表达可能是对细胞内毒性分子产生的一种适应性反应，但不伴有转录后过表达，可能没有功能影响。
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