Department of Neurology, Hangzhou Xixi Hospital Affiliated to Zhejiang University of Traditional Chinese Medicine, Hangzhou 310024, China.
J Healthc Eng. 2022 Apr 11;2022:5152761. doi: 10.1155/2022/5152761. eCollection 2022.
In order to investigate the effect of notoginsenoside Rg1 on cognitive function in 5FAD mice and the mechanism of Cx43 in improving cognitive function in mice, the methods 5FAD mice are selected as experimental animals and normal mice as healthy control. They were divided into three groups: healthy control group ( = 10), disease group ( = 10), and treatment group ( = 10, Panax notoginsenoside Rg 1150 mg/kg/d) for 2 months. Two months later, three groups of mice were subjected to classical water maze and Y-maze to test the cognitive ability of mice, and the correct response times and total response time were recorded. At the end of the cognitive function test, the mice were executed, and the brain tissues were taken. The expression of Cx43 protein and the changes of glial cells and neurons in the brain of the mice were analyzed at the cellular level. After treatment with Panax notoginseng saponin Rg 1150 mg/kg/d, it was found that the escape latency of mice in the treatment group was significantly lower than that in the disease group from the third day of training ( < 0.05); the time spent on the platform quadrant and the number of times crossing the platform in the treated mice were significantly increased, and the difference was statistically significant ( < 0.05); the expression of Cx43 protein in the brain of mice after treatment was significantly higher than that of mice in the disease group ( < 0.05). Panax notoginseng saponin Rg1 can effectively improve the cognitive function of 5FAD mice by increasing the secretion of Cx43 protein, thus increasing the reactivity of glial cells and neurons.
为了研究三七总皂苷 Rg1 对 5FAD 小鼠认知功能的影响及其通过 Cx43 改善小鼠认知功能的机制，选择 5FAD 小鼠作为实验动物，正常小鼠作为健康对照。将其分为三组：健康对照组（ = 10）、疾病组（ = 10）和治疗组（ = 10，给予三七总皂苷 Rg1 150 mg/kg/d），干预 2 个月。2 个月后，三组小鼠分别进行经典水迷宫和 Y 迷宫实验，以测试小鼠的认知能力，并记录正确反应次数和总反应时间。认知功能测试结束后处死小鼠，取脑组织，在细胞水平分析 Cx43 蛋白的表达以及小鼠脑内胶质细胞和神经元的变化。经 150 mg/kg/d 三七总皂苷 Rg1 治疗后发现，治疗组小鼠从第 3 天训练开始逃避潜伏期明显低于疾病组（ < 0.05）；治疗组小鼠在平台象限停留时间和穿越平台次数明显增加，差异有统计学意义（ < 0.05）；治疗组小鼠脑内 Cx43 蛋白表达明显高于疾病组（ < 0.05）。三七总皂苷 Rg1 可通过增加 Cx43 蛋白的分泌，有效改善 5FAD 小鼠的认知功能，从而提高胶质细胞和神经元的反应性。
