Nassar Muammar A Y, Eldien Heba M Saad, Tawab Hanem S Abdel, Saleem Tahia H, Omar Hossam M, Nassar Ahmed Y, Hussein Mahmoud Rezk Abdelwahed
Department of Zoology-Physiology, Faculty of Science, Assiut University, Assiut, Egypt.
Ultrastruct Pathol. 2012 Oct;36(5):343-55. doi: 10.3109/01913123.2012.685687.
Thermal tissue injury is partly mediated by reactive oxygen metabolites. Oxygen free radicals are contributory to local tissue damage following thermal injury and accordingly an interventional therapy using antioxidants may be beneficial. Copper nicotinate complex can scavenge reactive oxygen species (i.e., has antioxidant activity).
To examine time-related morphological and biochemical changes following skin thermal injury and their modulation by copper nicotinate complex.
An animal model composed of 80 albino rats was established. Ten rats (nonburn group) served as a control group. Seventy rats (burn group) were anesthetized, given a 10% total body surface area, full-thickness burn. Ten rats (from the postburn group) were sacrificed after 24 h (without treatment, i.e., untreated-burn group). The remaining rats were divided into three subgroups (20 rats, each) and were treated topically either with soft paraffin, moist exposed burn ointment (MEBO, a standard therapeutic treatment for burns), or copper nicotinate complex. Five animals from each subgroup were sacrificed every week over a period of 4 weeks. The morphological and biochemical changes were evaluated and compared among the different groups.
High levels of the plasma and skin nitiric oxide (marker of oxidative stress) were observed in the untreated-burn group. These levels were significantly low following the application of copper nicotinate complex. Low levels of plasma and skin superoxide dismutase (marker of oxidative stress) and plasma ceruloplasmin were observed in the untreated-burn group. These levels were significantly high following copper nicotinate complex treatment. The total and differential leukocyte counts were low following the onset of the thermal injury. They gradually returned to normal levels over a 4-week period following the application of MEBO or copper nicotinate complex. Compared to untreated-burn group, postburn-healing changes (resolution of the inflammatory reaction, reepithelization of the epidermis, angiogenesis, deposition of collagen fibers, and recovery of the subcellualr organelles) were significantly accelerated following the application of either MEBO or copper nicotinate complex.
Application of copper nicotinate complex was associated with improved healing of the thermal burns of the skin. The underlying molecular changes underlying these effects await further investigations.
热损伤组织部分是由活性氧代谢产物介导的。氧自由基会导致热损伤后局部组织损伤，因此使用抗氧化剂的介入性治疗可能有益。烟酸铜络合物能够清除活性氧（即具有抗氧化活性）。
研究皮肤热损伤后随时间变化的形态学和生化改变以及烟酸铜络合物对这些改变的调节作用。
建立了一个由80只白化大鼠组成的动物模型。10只大鼠（未烧伤组）作为对照组。70只大鼠（烧伤组）麻醉后给予10%体表面积的全层烧伤。10只大鼠（烧伤后组）在24小时后处死（未治疗，即未治疗烧伤组）。其余大鼠分为三个亚组（每组20只），分别局部涂抹软石蜡、湿润烧伤膏（MEBO，一种标准的烧伤治疗药物）或烟酸铜络合物。在4周的时间里，每周从每个亚组中处死5只动物。对不同组之间的形态学和生化改变进行评估和比较。
在未治疗烧伤组中观察到血浆和皮肤一氧化氮（氧化应激标志物）水平较高。应用烟酸铜络合物后，这些水平显著降低。在未治疗烧伤组中观察到血浆和皮肤超氧化物歧化酶（氧化应激标志物）水平以及血浆铜蓝蛋白水平较低。应用烟酸铜络合物治疗后，这些水平显著升高。热损伤发生后，白细胞总数和分类计数较低。应用MEBO或烟酸铜络合物后，在4周内它们逐渐恢复到正常水平。与未治疗烧伤组相比，应用MEBO或烟酸铜络合物后，烧伤后愈合变化（炎症反应消退、表皮再上皮化、血管生成、胶原纤维沉积和亚细胞器恢复）显著加速。
应用烟酸铜络合物与皮肤热烧伤愈合改善有关。这些作用背后的潜在分子变化有待进一步研究。
