Başak Pinar Y, Ağalar Fatih, Gültekin Fatih, Eroğlu Erol, Altuntaş Irfan, Ağalar Canan
Department of Dermatology, Süleyman Demirel University School of Medicine, Turkey.
Ulus Travma Acil Cerrahi Derg. 2003 Apr;9(2):96-101.
Oxygen - free radicals are generated during inflammatory reactions and cause tissue damage when overproduced. The wounds, especially burn injuries which comprise several events, result in generation of reactive oxygen species and impairment of cellular functions as in wound healing process. This experimental study was done in order to investigate whether 20% body surface area, third degree burn injury creates systemic impairment in wound healing. Additionally, our aim was to evaluate the effects of melatonin on incisional cutaneous wound healing.
Fifty adult Wistar-Albino rats were included in the study. A group of animals were subjected to dorsal burn injury followed by full-thickness midline skin incision, 2 cm in length on the abdominal region which was primarily sutured. Melatonin was administered on incisional wounds and breaking strength, hydroxyproline, thiobarbituric acid reactive substance values and antioxidant enzyme activities in the wounded tissue were determined at day 7; to examine firstly the influence of thermal injury on systemic wound healing and secondly, whether melatonin possesses improving effects.
No detrimental effect of 20% burn injury on unburned cutaneous incisional wound healing was determined. There was not any difference in breaking strength, hydroxyproline, thiobarbituric acid reactive substance and glutathione peroxidase values, except for significantly elevated catalase and superoxide dismutase activities in melatonin-treated animals comparing to the control group.
This preliminary study disclosed that exogenous melatonin, at a dose of 10mg/kg for two days, exerted few variation in antioxidant status during wound repair. Nevertheless, half-life of melatonin is short and further studies are required, to investigate longer duration or higher dosage of administration which may be beneficial for cutaneous wound healing.
炎症反应过程中会产生氧自由基，过量产生时会导致组织损伤。伤口，尤其是包含多个事件的烧伤，会在伤口愈合过程中产生活性氧并损害细胞功能。本实验研究旨在调查20%体表面积的三度烧伤是否会对伤口愈合造成全身损害。此外，我们的目的是评估褪黑素对切开的皮肤伤口愈合的影响。
50只成年Wistar - Albino大鼠纳入研究。一组动物先进行背部烧伤，然后在腹部进行2厘米长的全层中线皮肤切开，伤口进行一期缝合。在切开的伤口上给予褪黑素，并在第7天测定伤口组织的抗张强度、羟脯氨酸、硫代巴比妥酸反应物质值和抗氧化酶活性，以首先检查热损伤对全身伤口愈合的影响，其次检查褪黑素是否具有改善作用。
未发现20%烧伤对未烧伤的皮肤切开伤口愈合有不利影响。与对照组相比，褪黑素处理组动物的过氧化氢酶和超氧化物歧化酶活性显著升高，而抗张强度、羟脯氨酸、硫代巴比妥酸反应物质和谷胱甘肽过氧化物酶值没有差异。
这项初步研究表明，剂量为10mg/kg、连续两天给予的外源性褪黑素在伤口修复过程中对抗氧化状态影响不大。然而，褪黑素的半衰期较短，需要进一步研究来探讨更长给药时间或更高剂量给药是否对皮肤伤口愈合有益。
