在活体大鼠中成像膜运输的活体显微镜检查。
Intravital microscopy to image membrane trafficking in live rats.
作者信息
Masedunskas Andrius, Sramkova Monika, Parente Laura, Weigert Roberto
机构信息
Intracellular Membrane Trafficking Unit, Oral and Pharyngeal Cancer Branch, National Institute of Dental and Craniofacial Research, National Institute of Health, Bethesda, MD, USA.
出版信息
Methods Mol Biol. 2013;931:153-67. doi: 10.1007/978-1-62703-056-4_9.
Abstract
Intravital microscopy is a powerful tool that enables imaging various biological processes in live animals. Here, we describe a series of procedures designed to image subcellular structures, such as endosomes and secretory vesicles in the salivary glands (SGs) of live rats. To this aim, we used fluorescently labeled molecules and/or fluorescently tagged proteins that were transiently transfected in the live animal.
摘要
活体显微镜检查是一种强大的工具，可对活体动物体内的各种生物学过程进行成像。在这里，我们描述了一系列程序，旨在对活大鼠唾液腺（SGs）中的亚细胞结构（如内体和分泌囊泡）进行成像。为此，我们使用了在活体动物中瞬时转染的荧光标记分子和/或荧光标记蛋白。
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