Intracellular Membrane Trafficking Unit, Oral and Pharyngeal Cancer Branch, National Institute of Dental and Craniofacial Research, National Institute of Health, 30 Convent drive, Room 303A, Bethesda, MD 20892-4340, USA.
Adv Drug Deliv Rev. 2011 Jan-Feb;63(1-2):119-28. doi: 10.1016/j.addr.2010.09.009. Epub 2010 Oct 7.
The technical developments in the field of non-linear microscopy have made intravital microscopy one of the most successful techniques for studying physiological and pathological processes in live animals. Intravital microscopy has been utilized to address many biological questions in basic research and is now a fundamental tool for preclinical studies, with an enormous potential for clinical applications. The ability to dynamically image cellular and subcellular structures combined with the possibility to perform longitudinal studies have empowered investigators to use this discipline to study the mechanisms of action of therapeutic agents and assess the efficacy on their targets in vivo. The goal of this review is to provide a general overview of the recent advances in intravital microscopy and to discuss some of its applications in preclinical studies.
非线性显微镜领域的技术发展使活体显微镜成为研究活体动物生理和病理过程最成功的技术之一。活体显微镜已被用于解决基础研究中的许多生物学问题,并且现在是临床前研究的基本工具,具有巨大的临床应用潜力。动态成像细胞和亚细胞结构的能力以及进行纵向研究的可能性,使研究人员能够利用该学科研究治疗剂的作用机制,并评估其在体内靶标上的疗效。本文的目的是提供活体显微镜的最新进展的概述,并讨论其在临床前研究中的一些应用。