Can Dis Wkly Rep. 1990 Jan 27;16(4):17-8.
In late November 1989, Ebola virus was isolated from cynomolgus monkeys (Macaca fascicularis) imported into the United States from the Philippines via Amsterdam and New York. During quarantine in a primate facility in Virginia, numerous macaques died, some with findings consistent with simian hemorrhagic fever (SHF). The US Army Medical Research Institute of Infectious Diseases tested 10 animals and, from 3, isolated SHF from tissues and serum; however, 5 other animals of the 10 tested were positive for Ebola virus. Monkeys from a later shipment quarantined in a second room also had unusually high mortality and were tested by a rapid antigen detection enzyme-linked immunosorbent assay. Ebola viral antigen was detected in serum and/or tissues from 7 of these monkeys. Primary liver material from animals in both rooms exhibited particles with typical filovirus morphology by electron microscopy and Ebola virus antigen by immunohistochemistry.
1989年11月下旬，从经阿姆斯特丹和纽约从菲律宾进口到美国的食蟹猴（猕猴）中分离出埃博拉病毒。在弗吉尼亚州的一个灵长类动物设施隔离期间，大量猕猴死亡，一些猕猴的症状与猴出血热（SHF）相符。美国陆军传染病医学研究所对10只动物进行了检测，从3只动物的组织和血清中分离出猴出血热病毒；然而，在接受检测的10只动物中，另外5只对埃博拉病毒呈阳性反应。后来一批在第二个房间隔离的猴子也有异常高的死亡率，并通过快速抗原检测酶联免疫吸附试验进行了检测。在这些猴子中的7只的血清和/或组织中检测到了埃博拉病毒抗原。两个房间动物的原代肝脏材料通过电子显微镜显示出具有典型丝状病毒形态的颗粒，并通过免疫组织化学显示出埃博拉病毒抗原。
