Aquatic Contaminants Research Division, Water, Science, and Technology Directorate, Environment Canada, Burlington, Ontario, Canada.
Environ Toxicol Chem. 2012 Dec;31(12):2788-97. doi: 10.1002/etc.2023. Epub 2012 Oct 24.
Whole-body polychlorinated biphenyls (ΣPCBs) and plasma hydroxylated PCBs (OH-PCBs) concentrations were determined in brown bullhead (Ameiurus nebulosus) from Wheatley Harbour, Ontario, Canada. Elevated ΣPCBs in Wheatley Harbour are suspected to have originated from industrial waste disposal and/or discharges from nearby fish processing through discarding of fish remains. Mean ΣPCB concentrations in brown bullhead from Wheatley Harbour were approximately 250 ng/g wet weight compared with approximately 40 ng/g wet weight for brown bullhead from the reference sites, Hillman Marsh and Turkey Creek (both in Ontario, Canada). A significant relationship was found between the concentrations of non-ortho and mono-ortho PCB concentrations (toxic equivalents) and liver mixed-function oxygenase in brown bullhead (r = 0.74, p < 0.001). Plasma OH-PCB concentrations were greater in Wheatley Harbour brown bullhead than in those from Hillman Marsh (3.6 vs 1.5 ng/g wet wt, p < 0.01), and were detected infrequently in those from Turkey Creek (0.1 ng/g wet wt, n = 2). The OH-PCB congeners most frequently detected were 4'-OH-CB172, 3'-OH-CB180, 4-OH-CB187, 4-OH-CB146, 3-OH-CB138, and 4-OH-CB130, which are structurally similar to the thyroid hormones. To test the hypothesis of fish waste as the cause of the observed PCB contamination of Wheatley Harbour brown bullhead, a principal component analysis (PCA) was used to compare the brown bullhead PCB congener data with equivalent data for Lake Erie walleye, Lake Erie sediment, and industrial Aroclor mixtures. The relative proportions of each Aroclor mixture were estimated using the conjugated gradient method. The high similarity between the congener signatures for Lake Erie walleye and Wheatley Harbour brown bullhead supports the hypothesis of contamination from the fish processing industry.
对来自加拿大安大略省惠特利港的褐鳜（Ameiurus nebulosus）进行了全身多氯联苯（ΣPCBs）和血浆羟基化多氯联苯（OH-PCBs）浓度的测定。惠特利港的ΣPCBs 含量升高，据推测是由于工业废物处理和/或附近鱼类加工厂丢弃鱼类残余物而排放的。惠特利港褐鳜的平均ΣPCB 浓度约为 250ng/g 湿重，而来自参考地点希尔曼沼泽和火鸡溪（均位于加拿大安大略省）的褐鳜的平均ΣPCB 浓度约为 40ng/g 湿重。褐鳜肝脏混合功能氧化酶与非邻位和单邻位 PCB 浓度（毒性当量）之间存在显著关系（r=0.74，p<0.001）。惠特利港褐鳜的血浆 OH-PCB 浓度高于希尔曼沼泽的褐鳜（3.6 与 1.5ng/g 湿重，p<0.01），而在火鸡溪的褐鳜中很少检测到（0.1ng/g 湿重，n=2）。最常检测到的 OH-PCB 同系物是 4'-OH-CB172、3'-OH-CB180、4-OH-CB187、4-OH-CB146、3-OH-CB138 和 4-OH-CB130，它们的结构与甲状腺激素相似。为了检验鱼类废物是导致惠特利港褐鳜 PCB 污染的原因这一假说，使用主成分分析（PCA）将褐鳜 PCB 同系物数据与安大略湖湖鳜、安大略湖沉积物和工业 Aroclor 混合物的等效数据进行了比较。使用共轭梯度法估计了每种 Aroclor 混合物的相对比例。安大略湖湖鳜和惠特利港褐鳜的同系物特征高度相似，支持了鱼类加工业污染的假说。
