Tillyer C
Department of Chemical Pathology, Royal Marsden Hospital, London, U.K.
Clin Chem. 1990 Feb;36(2):307-12.
I describe a general technique, called "two-parameter calibration," which allows precise determination of analyte from non-monotonic calibration plots and the calibration of immunoturbidimetric assays in antigen excess. Using three-dimensional calibration plots and relative-sensitivity curves, two optimal parameters may be selected from a number of possible options by using criteria presented here. Choosing two different values of delta At1-t2, the change in absorbance from time t1 to t2--as the reaction parameters in an immunoturbidimetric assay for albumin, I have optimized the choice of time interval for two-parameter calibration and extended the working range of the assay by three- to fourfold. The albumin assay shows excellent agreement of observed and expected values (r = 0.996) and also with results of a routine kinetic dye-binding method used on diluted plasma samples (r = 0.970).
我描述了一种称为“双参数校准”的通用技术，它能够从非单调校准曲线中精确测定分析物，并可用于抗原过量情况下免疫比浊法的校准。利用三维校准曲线和相对灵敏度曲线，可以根据本文提出的标准从多个可能选项中选择两个最佳参数。在白蛋白免疫比浊测定中，选择两个不同的吸光度变化值（从时间t1到t2的吸光度变化，即δAt1-t2）作为反应参数，我优化了双参数校准的时间间隔选择，并将该测定的工作范围扩大了三到四倍。白蛋白测定显示观测值与预期值高度吻合（r = 0.996），并且与用于稀释血浆样本的常规动力学染料结合法的结果也高度吻合（r = 0.970）。
