Kim Joo Yeon, Shaker Mohammed R, Lee Ju-Hyun, Lee Boram, Kim Hyun, Sun Woong
Department of Anatomy, Korea University College of Medicine, Seoul, South Korea.
Anim Cells Syst (Seoul). 2017 May 18;21(3):152-159. doi: 10.1080/19768354.2017.1324522. eCollection 2017.
Neural stem cells (NSCs) in the adult subventricular zone (SVZ) are regionally specified and have distinct molecular gene expression signatures. Recently, we identified the subcallosal zone (SCZ) as a novel brain region where adult NSCs maintain and spontaneously produce neuroblasts. In an attempt to isolate genes specifically expressed in the SCZ or SVZ, microarray analyses of their differentially expressing transcripts were done. The comparison between neurospheres generated from SVZ and SCZ revealed differential expression >1.5-fold in two groups in only 83 genes, representing <0.03% of the genes examined, suggesting that these two populations are largely similar. The differential expression patterns SCZ and SVZ genes were confirmed by RT-PCR and Western blots. The selective expressions of two genes (, ) in SVZ-NSCs were further confirmed by immunohistochemistry. These molecular markers could be useful for further molecular and cellular characterization of NSCs.
成年脑室下区（SVZ）的神经干细胞（NSCs）具有区域特异性，且有独特的分子基因表达特征。最近，我们将胼胝体下区（SCZ）确定为一个新的脑区，成年神经干细胞在此区域维持并自发产生神经母细胞。为了分离在SCZ或SVZ中特异性表达的基因，我们对它们差异表达的转录本进行了微阵列分析。对从SVZ和SCZ产生的神经球进行比较发现，两组中差异表达>1.5倍的基因仅83个，占所检测基因的<0.03%，这表明这两个群体在很大程度上是相似的。SCZ和SVZ基因的差异表达模式通过逆转录聚合酶链反应（RT-PCR）和蛋白质免疫印迹法得到了证实。通过免疫组织化学进一步证实了两个基因（，）在SVZ神经干细胞中的选择性表达。这些分子标记物可能有助于对神经干细胞进行进一步的分子和细胞特征分析。
