口服脂肪对人体血浆神经降压素及神经降压素片段水平的影响。通过高压液相色谱法进行表征。

Effect of oral fat on plasma levels of neurotensin and neurotensin fragments in humans. Characterization by high-pressure liquid chromatography.

作者信息

Draviam E J, Upp J R, Greeley G H, Townsend C M, Thompson J C

机构信息

Department of Surgery, University of Texas Medical Branch, Galveston 77550.

出版信息

Dig Dis Sci. 1990 Feb;35(2):200-4. doi: 10.1007/BF01536763.

DOI:10.1007/BF01536763

PMID:2302977

Abstract

The effect of ingestion of fat (Lipomul 1 g/kg) on the circulating levels of neurotensin (NT1-13) and amino-terminal fragments (NT1-8, NT1-11) and carboxy-terminal fragment (NT8-13) of NT were investigated in six healthy male volunteers. NT and NT fragments were extracted from plasma collected at 0, 15, 30, and 60 min after ingestion of fat, and the plasma levels of NT1-13 and NT fragments were characterized using high-pressure liquid chromatography and radioimmunoassay techniques. Significant elevations of plasma levels of NT1-8, NT1-11, and NT1-13 were observed at 15, 30, and 60 min after fat ingestion. The maximum elevations were 273% for NT1-8, 234% for NT1-11, and 54% for NT1-13. NT8-13 levels failed to rise significantly when compared to basal levels. These findings indicate that both the amino-terminal and carboxy-terminal fragments of NT are either released along with intact NT or are formed as metabolites from NT1-13 in response to ingestion of fat in man.

摘要

在六名健康男性志愿者中，研究了摄入脂肪（Lipomul，1 g/kg）对神经降压素（NT1-13）及其氨基末端片段（NT1-8、NT1-11）和羧基末端片段（NT8-13）循环水平的影响。在摄入脂肪后0、15、30和60分钟采集血浆，从中提取NT和NT片段，并使用高压液相色谱和放射免疫测定技术对NT1-13和NT片段的血浆水平进行表征。在摄入脂肪后15、30和60分钟时，观察到NT1-8、NT1-11和NT1-13的血浆水平显著升高。NT1-8的最大升高幅度为273%，NT1-11为234%，NT1-13为54%。与基础水平相比，NT8-13水平未显著升高。这些发现表明，NT的氨基末端和羧基末端片段要么与完整的NT一起释放，要么是人体摄入脂肪后由NT1-13形成的代谢产物。