神经降压素的放射免疫测定以及大鼠和犬肠道神经降压素的分布与浓度

Radioimmunoassay of neurotensin and the distribution and concentration of gut neurotensin in rat and dog.

作者信息

Iwasaki Y, Ito S, Shibata A

出版信息

Tohoku J Exp Med. 1980 Feb;130(2):129-37. doi: 10.1620/tjem.130.129.

DOI:10.1620/tjem.130.129

Abstract

A specific radioimmunoassay (RIA) for neurotensin (NT) has been developed. Four antisera of high avidity were raised in 4 rabbits immunized with synthetic NT tridecapeptide coupled with bovine serum albumin via glutaraldehyde. NT was labeled with Na125I by means of modified Hunter-Greenwood's chloramine-T method. Sensitivity of the RIA was 100 pg/ml. Cross-reactivity of antiserum to other peptides was not observed. Using the RIA, the distribution and concentration of gastrointestinal (GI)-NT have been determined in acid-acetone extracts of rat and dog GI tissues. RIA using four kinds of antisera similarly detected higher concentrations in jejuno-ileal sections than in other regions. Although the distribution of GI-NT was quite similar between rat and dog, its concentration showed species specific difference.

摘要

已开发出一种针对神经降压素（NT）的特异性放射免疫分析（RIA）方法。用戊二醛将合成的NT十三肽与牛血清白蛋白偶联后，对4只兔子进行免疫，获得了4种高亲和力的抗血清。采用改良的Hunter-Greenwood氯胺-T法用Na125I对NT进行标记。该RIA的灵敏度为100 pg/ml。未观察到抗血清与其他肽的交叉反应。使用该RIA，已测定了大鼠和犬胃肠道（GI）组织的酸-丙酮提取物中胃肠道NT的分布和浓度。使用四种抗血清的RIA同样检测到空肠-回肠段中的浓度高于其他区域。尽管大鼠和犬之间胃肠道NT的分布非常相似，但其浓度存在种属特异性差异。