Iwasaki Y, Ito S, Shibata A
Tohoku J Exp Med. 1980 Feb;130(2):129-37. doi: 10.1620/tjem.130.129.
A specific radioimmunoassay (RIA) for neurotensin (NT) has been developed. Four antisera of high avidity were raised in 4 rabbits immunized with synthetic NT tridecapeptide coupled with bovine serum albumin via glutaraldehyde. NT was labeled with Na125I by means of modified Hunter-Greenwood's chloramine-T method. Sensitivity of the RIA was 100 pg/ml. Cross-reactivity of antiserum to other peptides was not observed. Using the RIA, the distribution and concentration of gastrointestinal (GI)-NT have been determined in acid-acetone extracts of rat and dog GI tissues. RIA using four kinds of antisera similarly detected higher concentrations in jejuno-ileal sections than in other regions. Although the distribution of GI-NT was quite similar between rat and dog, its concentration showed species specific difference.
已开发出一种针对神经降压素（NT）的特异性放射免疫分析（RIA）方法。用戊二醛将合成的NT十三肽与牛血清白蛋白偶联后，对4只兔子进行免疫，获得了4种高亲和力的抗血清。采用改良的Hunter-Greenwood氯胺-T法用Na125I对NT进行标记。该RIA的灵敏度为100 pg/ml。未观察到抗血清与其他肽的交叉反应。使用该RIA，已测定了大鼠和犬胃肠道（GI）组织的酸-丙酮提取物中胃肠道NT的分布和浓度。使用四种抗血清的RIA同样检测到空肠-回肠段中的浓度高于其他区域。尽管大鼠和犬之间胃肠道NT的分布非常相似，但其浓度存在种属特异性差异。
