Wu Fei-yi, Heng Kee Khiang, Salleh Rohani Binte, Soh Teck Guan, Lee Jing Jing, Mah Joanna, Linn Yeh Ching, Loh Yvonne, Hwang William, Tan Lip Kun, Law Ping, Goh Yeow Tee
Department of Haematology, Singapore General Hospital, Singapore.
Transfus Apher Sci. 2012 Dec;47(3):345-50. doi: 10.1016/j.transci.2012.07.015. Epub 2012 Sep 30.
Peripheral blood stem cells (PBSC) have become the most common source of hematopoietic cells for allogeneic or autologous blood and marrow transplantation (BMT). We performed an evaluation of PBSC collections using three different apheresis systems in two major transplantation centers in Singapore. Patients undergoing autologous BMT and donors collecting for allogeneic BMT were harvested using the COBE Spectra, Haemonetics MCS+, or Baxter Amicus. There were 99 Spectra collections (61 were autologous), 81 MCS+ collections (35 were autologous) and 38 Amicus collections (33 were autologous). Our data shows that the Amicus not only processed larger peripheral blood volumes but also yielded larger PBSC volume (P-value<0.05). In terms of PBSC products, the Spectra produced more WBC, WBC/liter blood processed, and WBC/kg (P-value<0.05). The Spectra and MCS+ produced comparable amount of CD34+ cells. Amicus collected 50% less platelets compared to Spectra and MCS+. The total CD34+ cells in the PBSC products was linearly correlated to the circulating CD34+ cells using Spectra, MCS+, and Amicus. Our results suggest that, compared to MCS+ and Amicus, collecting PBSC using the COBE Spectra can produce more WBC with a similar number of CD34+ cells. With a linear correlation of circulating CD34+ cells to the total CD34+ cells in the products, the availability of an automated procedure, no rotating seal, and a small extracorporeal volume, the Spectra appears to be the preferred machine for PBSC collection.
外周血干细胞（PBSC）已成为同种异体或自体血液及骨髓移植（BMT）中最常见的造血细胞来源。我们在新加坡的两个主要移植中心使用三种不同的血液分离系统对PBSC采集情况进行了评估。接受自体BMT的患者以及为同种异体BMT采集的供者，使用COBE Spectra、Haemonetics MCS +或百特Amicus进行采集。共有99次使用Spectra采集（其中61次为自体采集），81次使用MCS +采集（其中35次为自体采集），38次使用Amicus采集（其中33次为自体采集）。我们的数据表明，Amicus不仅能处理更大体积的外周血，而且能获得更大体积的PBSC（P值<0.05）。就PBSC产品而言，Spectra产生的白细胞更多，每升处理血液中的白细胞以及每千克体重的白细胞更多（P值<0.05）。Spectra和MCS +产生的CD34 +细胞数量相当。与Spectra和MCS +相比，Amicus采集的血小板减少了50%。使用Spectra、MCS +和Amicus时，PBSC产品中的总CD34 +细胞与循环中的CD34 +细胞呈线性相关。我们的结果表明，与MCS +和Amicus相比，使用COBE Spectra采集PBSC能在产生相似数量CD34 +细胞的情况下产生更多白细胞。鉴于循环中的CD34 +细胞与产品中总CD34 +细胞呈线性相关，且具有自动化程序、无旋转密封以及体外循环体积小等特点，Spectra似乎是PBSC采集的首选机器。
