Setia Rasika Dhawan, Arora Satyam, Handoo Anil, Dadu Tina, Choudhary Dharma, Sharma Sajeev Kumar, Kharya Gaurav, Khandelwal Vipin, Sachdeva Prerna, Doval Divya, Bakliwal Anamika, Kapoor Meenu, Bajaj Shalu, Bachchas Virendra, Singh Praveen
Department of Transfusion Medicine, BLK Super Speciality Hospital, Delhi, India.
Department of Transfusion Medicine, BLK Super Speciality Hospital, Delhi, India.
Transfus Apher Sci. 2017 Jun;56(3):439-444. doi: 10.1016/j.transci.2017.04.002. Epub 2017 Apr 24.
Most common source of stem cell graft for both autologous and allogenic haematopoietic transplants are peripheral blood haematopoietic progenitor stem cells. Adequate collection of the CD34+ cells and safety of the allogenic donor during the leukapheresis are of prime importance to an apheresis physician. Our retrospective analysis is a comparison between of two platforms namely, COBE Spectra and Amicus, for CD34+ mononuclear cell collection.
The study included the data of GSCF (Granulocyte-Colony-Stimulating Factor) mobilized allogenic PBSC collections at our centre from January 2015 to June 2016. The apheresis platforms used were COBE Spectra and Amicus. Blood cell counts were done using LH750 Beckman Coulter (Florida, Miami, USA). CD45+ & CD34+ cell counts were done using BD FACS Canto-II Flow-Cytometer by ISHAGE guidelines.
A total of 170 PBSC (100 COBE Spectra & 70 Amicus) harvests were done on 143 donors, of which 116 completed the collection in a single session and 27 required a second session. Demographic details and pre harvest peripheral blood counts for both the groups did not show any statistical differences. Amicus processed higher blood volume with higher ACD exposure and procedure time compared to COBE Spectra. Higher platelets loss was with COBE Spectra harvests with higher product volumes collection. Collection efficiency (CE2), collection ratio, CD34+ cells dose was similar on both the platforms. RBC contamination, absolute lymphocyte and monocytes counts were significantly higher with Amicus harvest product compared with COBE Spectra. A total of 14 (8.2%; citrate toxicity) adverse reactions were reported out of 170 allogenic PBSC collections.
DISCUSSION/CONCLUSION: Our study suggests that both Amicus and COBE Spectra platforms offer comparable results for allogenic PBSC collections. Amicus offers a concentrated PBSC product with lesser volume and platelets loss but higher RBC contamination.
对于自体和异体造血移植而言，外周血造血祖干细胞是干细胞移植最常见的来源。对于单采医生来说，在白细胞单采过程中充分采集CD34+细胞以及异体供者的安全性至关重要。我们的回顾性分析是对两个平台（即COBE Spectra和Amicus）采集CD34+单核细胞进行比较。
该研究纳入了2015年1月至2016年6月在我们中心进行的GSCF（粒细胞集落刺激因子）动员的异体PBSC采集数据。所使用的单采平台为COBE Spectra和Amicus。血细胞计数采用LH750贝克曼库尔特血细胞分析仪（美国佛罗里达州迈阿密）进行。CD45+和CD34+细胞计数采用BD FACS Canto-II流式细胞仪按照ISHAGE指南进行。
共对143名供者进行了170次PBSC采集（100次使用COBE Spectra，70次使用Amicus），其中116名在单次采集时完成，27名需要进行第二次采集。两组的人口统计学细节和采集前外周血计数均未显示出任何统计学差异。与COBE Spectra相比，Amicus处理的血量更多，ACD暴露量和操作时间更长。COBE Spectra采集时血小板损失更高，采集的产品体积更大。两个平台的采集效率（CE2）、采集比例、CD34+细胞剂量相似。与COBE Spectra相比，Amicus采集的产品中红细胞污染、绝对淋巴细胞和单核细胞计数明显更高。在170次异体PBSC采集中，共报告了14例（8.2%；枸橼酸盐毒性）不良反应。
讨论/结论：我们的研究表明，Amicus和COBE Spectra平台在异体PBSC采集中提供了可比的结果。Amicus提供的PBSC产品浓度更高，体积和血小板损失更小，但红细胞污染更高。
