Department of Otorhinolaryngology-Head and Neck Surgery, Universidade Federal de São Paulo, Federal University of São Paulo, São Paulo, Brazil.
Otol Neurotol. 2012 Dec;33(9):1625-9. doi: 10.1097/MAO.0b013e31826939e4.
The establishment of reliable methods for functional assessment in experimental models of peripheral nerve regeneration is crucial.
We present a straightforward method for video analysis of the eye blink reflex in a model of facial nerve damage in a nonhuman primate (Callithrix sp.).
Our 6-level dynamic analysis demonstrated good reproducibility between independent observers, as measured by Cohen's kappa index. Our static analysis, which was based on 4 semiautomated metric parameters, showed low correlation during the early stage of facial movement recovery (the first and second weeks), but the correlation was excellent during the later stage of recovery (the third and fourth weeks).
Altogether, our results establish a viable and readily accessible method with good reproducibility and correlation for the analysis of functional facial nerve recovery in an experimental model and based on video images of the eye blink reflex.
建立可靠的方法来评估周围神经再生的实验模型中的功能是至关重要的。
我们提出了一种简单的方法，用于分析非人灵长类动物（Callithrix sp.）面神经损伤模型中的眨眼反射的视频。
我们的 6 级动态分析显示，独立观察者之间的重复性良好，用 Cohen's kappa 指数来衡量。我们的静态分析基于 4 个半自动度量参数，在面神经运动恢复的早期（第 1 周和第 2 周）相关性较低，但在恢复的后期（第 3 周和第 4 周）相关性极好。
总的来说，我们的结果建立了一种可行的、易于获取的方法，具有良好的可重复性和相关性，可用于分析实验模型中面神经功能恢复的情况，并基于眨眼反射的视频图像。
