State Key Laboratory of Environmental Aquatic Chemistry, Beijing, China.
Water Sci Technol. 2012;66(11):2402-9. doi: 10.2166/wst.2012.448.
Resin fractionation is the most widely used technique to isolate and characterize natural organic matter (NOM) based on its hydrophobicity and hydrophilicity, however, it is also recognized as a time consuming technique. This paper describes the use of reverse phase high performance liquid chromatography (RPHPLC) as a rapid assessment technique to determine the hydrophobicity/hydrophilicity of NOM. The optimum column separation condition was achieved and without the need for concentrating the sample prior to analysis and with good reproducibility of the peak retention time and the peak area. The characterization results were further compared with the traditional resin fractionation technique using DAX-8 and XAD-4 resins. The results demonstrated that the polarities defined by the two methods were different but consistent and also that the fractions absorbed onto XAD-4 were less hydrophobic than those absorbed onto DAX-8. The difference in definition between resin fractionation and RPHPLC were further investigated.
树脂分馏是最广泛使用的技术，用于根据疏水性和亲水性分离和表征天然有机物（NOM），然而，它也被认为是一种耗时的技术。本文描述了反相高效液相色谱（RPHPLC）作为一种快速评估技术，用于确定 NOM 的疏水性/亲水性。实现了最佳柱分离条件，并且在分析之前不需要浓缩样品，并且峰保留时间和峰面积具有良好的重现性。进一步将表征结果与使用 DAX-8 和 XAD-4 树脂的传统树脂分馏技术进行了比较。结果表明，两种方法定义的极性不同但一致，并且吸收到 XAD-4 上的部分比吸收到 DAX-8 上的部分疏水性更小。进一步研究了树脂分馏和 RPHPLC 之间的定义差异。
