School of Chemistry & Ryan Institute, National University of Ireland Galway, Ireland.
Phys Chem Chem Phys. 2012 Nov 14;14(42):14667-72. doi: 10.1039/c2cp42089a. Epub 2012 Oct 2.
Co-immobilisation approaches for preparation of glucose-oxidising films of [Os(2,2'-bipyridine)(2)(poly-vinylimidazole)(10)Cl] and glucose oxidase on glassy carbon electrodes are compared. Electrodes prepared by crosslinking using glutaraldehyde vapour, without and with a NaBH(4) reduction, provide higher glucose oxidation current than those prepared using a well-established diepoxide method. Addition of multi walled carbon nanotubes to the film deposition solutions produces an enhanced glucose oxidation current density of 5 mA cm(-2) at 0.35 V vs. Ag/AgCl, whilst improving the operational stability of the current signal. Carbon nanotube, glutaraldehyde vapour crosslinked, films on electrodes, reduced by NaBH(4), retain 77% of initial catalytic current over 24 hours of continuous amperometric testing in a 37 °C, 50 mM phosphate buffer solution containing 150 mM NaCl and 100 mM glucose. Potential application of this approach to implantable enzymatic biofuel cells is demonstrated by production of glucose oxidation currents, under pseudo-physiological conditions, using mediating films with lower redox potentials.
比较了[Os(2,2'-联吡啶)(2)(聚乙烯基咪唑)(10)Cl]和葡萄糖氧化酶在玻碳电极上共固定化制备葡萄糖氧化膜的方法。用戊二醛蒸气交联、不还原和用 NaBH(4)还原制备的电极提供的葡萄糖氧化电流高于用成熟的二环氧化物方法制备的电极。将多壁碳纳米管添加到膜沉积溶液中可产生增强的葡萄糖氧化电流密度，在 37°C、50 mM 磷酸盐缓冲溶液中含有 150 mM NaCl 和 100 mM 葡萄糖时，在 0.35 V 对 Ag/AgCl 的情况下为 5 mA cm(-2)，同时提高电流信号的操作稳定性。在含有 150 mM NaCl 和 100 mM 葡萄糖的 37°C、50 mM 磷酸盐缓冲溶液中，经过 24 小时连续安培测试，在电极上用 NaBH(4)还原的碳纳米管、戊二醛蒸气交联的薄膜保留了初始催化电流的 77%。通过使用具有较低氧化还原电位的介体膜在拟生理条件下产生葡萄糖氧化电流，证明了这种方法在可植入酶生物燃料电池中的潜在应用。
