Wang Youdan, Joshi Pratixa P, Hobbs Kevin L, Johnson Matthew B, Schmidtke David W
University of Oklahoma Bioengineering Center, School of Chemical, Biological and Materials Engineering, Homer L. Dodge Department of Physics and Astronomy, University of Oklahoma, 100 East Boyd, Norman, Oklahoma 73019, USA.
Langmuir. 2006 Nov 7;22(23):9776-83. doi: 10.1021/la060857v.
In this study, we describe the construction of glucose biosensors based on an electrostatic layer-by-layer (LBL) technique. Gold electrodes were initially functionalized with negatively charged 11-mercaptoundecanoic acid followed by alternate immersion in solutions of a positively charged redox polymer, poly[(vinylpyridine)Os(bipyridyl)2Cl(2+/3+)], and a negatively charged enzyme, glucose oxidase (GOX), or a GOX solution containing single-walled carbon nanotubes (SWNTs). The LBL assembly of the multilayer films were characterized by UV-vis spectroscopy, ellipsometry, and cyclic voltammetry, while characterization of the single-walled nanotubes was performed with transmission electron microscopy, Raman spectroscopy, thermogravimetric analysis, and X-ray photoelectron spectroscopy. When the GOX solution contained single-walled carbon nanotubes (GOX-SWNTs), the oxidation peak currents during cyclic voltammetry increased 1.4-4.0 times, as compared to films without SWNTs. Similarly the glucose electro-oxidation current also increased (6-17 times) when SWNTs were present. By varying the number of multilayers, the sensitivity of the sensors could be controlled.
在本研究中，我们描述了基于静电层层（LBL）技术构建葡萄糖生物传感器的过程。金电极首先用带负电荷的11-巯基十一烷酸进行功能化处理，随后交替浸入带正电荷的氧化还原聚合物聚[（乙烯基吡啶）Os（联吡啶）2Cl（2+/3+）]溶液和带负电荷的酶葡萄糖氧化酶（GOX）溶液，或含有单壁碳纳米管（SWNTs）的GOX溶液中。多层膜的LBL组装通过紫外可见光谱、椭偏仪和循环伏安法进行表征，而单壁纳米管的表征则通过透射电子显微镜、拉曼光谱、热重分析和X射线光电子能谱进行。当GOX溶液含有单壁碳纳米管（GOX-SWNTs）时，与不含SWNTs的膜相比，循环伏安法期间的氧化峰电流增加了1.4至4.0倍。同样，当存在SWNTs时，葡萄糖电氧化电流也增加了（6至17倍）。通过改变多层的数量，可以控制传感器的灵敏度。
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