Kou Xing, Lei Jiandu, Geng Liyuan, Deng Hongquan, Jiang Qiying, Zhang Guifeng, Ma Guanghui, Su Zhiguo
State Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100190, PR China.
J Nanosci Nanotechnol. 2012 Sep;12(9):7388-94. doi: 10.1166/jnn.2012.6581.
Preparation of uniform size molecularly imprinted nanospheres for erythromycin with good selectivity and high binding capacity by precipitation polymerization were presented, in which erythromycin, methacrylic acid and ethylene glycol dimethacrylate are used as template molecule, functional monomer and cross-linker, respectively. The synthesis conditions of molecularly imprinted nanospheres were optimized and the optimal molar ratio of erythromycin to functional monomer is 1:3. The molecularly imprinted polymers were characterized by scanning electron microscope, laser particle size analyzer and BET, respectively. The results suggested that molecularly imprinted nanospheres for erythromycin exhibited spherical shape and good monodispersity. Selectivity analysis indicated that the imprinted nanospheres could specifically recognize erythromycin from its structure analogues. Furthermore, adsorption kinetics and adsorption isotherm of the imprinted nanospheres were employed to investigate the binding characteristics of the imprinted nanospheres. The results showed that the imprinted nanospheres have high adsorption capacity for erythromycin, and the maximum theoretical static binding capacity is up to 267.0188 mg g(-1).
介绍了通过沉淀聚合法制备具有良好选择性和高结合容量的均匀尺寸的红霉素分子印迹纳米球，其中分别使用红霉素、甲基丙烯酸和乙二醇二甲基丙烯酸酯作为模板分子、功能单体和交联剂。优化了分子印迹纳米球的合成条件，红霉素与功能单体的最佳摩尔比为1:3。分别用扫描电子显微镜、激光粒度分析仪和BET对分子印迹聚合物进行了表征。结果表明，红霉素分子印迹纳米球呈球形，具有良好的单分散性。选择性分析表明，印迹纳米球能够从其结构类似物中特异性识别红霉素。此外，采用印迹纳米球的吸附动力学和吸附等温线来研究印迹纳米球的结合特性。结果表明，印迹纳米球对红霉素具有较高的吸附容量，最大理论静态结合容量高达267.0188 mg g(-1)。
