单链 DNA 结合蛋白 SSB 对大肠杆菌 DNA 损伤诱导 DNA 解旋酶 DinG 的刺激作用。
Stimulation of Escherichia coli DNA damage inducible DNA helicase DinG by the single-stranded DNA binding protein SSB.
机构信息
Department of Biological Sciences, Louisiana State University, Baton Rouge, LA 70803, USA.
出版信息
FEBS Lett. 2012 Nov 2;586(21):3825-30. doi: 10.1016/j.febslet.2012.09.032. Epub 2012 Oct 1.
Abstract
Escherichia coli DNA damage inducible protein DinG is a superfamily II DNA helicase and is closely related to human DNA helicase XPD. Here, we report that E. coli single-stranded DNA binding protein (SSB) is able to form a stable protein complex with DinG and to stimulate the DinG DNA helicase activity. An SSB mutant that retains the single-stranded DNA binding activity but fails to form a protein complex with DinG becomes a potent inhibitor for the DinG DNA helicase, suggesting that E. coli wild-type SSB stimulates the DinG DNA helicase via specific protein-protein interaction.
摘要
大肠杆菌 DNA 损伤诱导蛋白 DinG 是超家族 II DNA 解旋酶，与人类 DNA 解旋酶 XPD 密切相关。在这里，我们报告大肠杆菌单链 DNA 结合蛋白（SSB）能够与 DinG 形成稳定的蛋白复合物，并刺激 DinG DNA 解旋酶活性。一种保留单链 DNA 结合活性但不能与 DinG 形成蛋白复合物的 SSB 突变体成为 DinG DNA 解旋酶的有效抑制剂，这表明大肠杆菌野生型 SSB 通过特定的蛋白-蛋白相互作用刺激 DinG DNA 解旋酶。
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