Der-Balin G P, Gomez B, Masino R S, Parce J W
Molecular Devices Corporation, Menlo Park, CA 94025.
J Immunol Methods. 1990 Feb 9;126(2):281-5. doi: 10.1016/0022-1759(90)90161-n.
A method to determine the number of biotin moieties attached to a protein has been developed based on quenching the natural fluorescence of avidin or streptavidin by biotin. The assay consists of titrating the number of biotin combining sites on streptavidin/avidin before and after adding the biotinylated protein. With this method only those biotin moieties capable of binding to streptavidin/avidin are detected. The assay is simple and sensitive, requiring only 1-10 micrograms of biotinylated protein per determination.
基于生物素对抗生物素蛋白或链霉抗生物素蛋白天然荧光的猝灭作用，已开发出一种测定与蛋白质相连的生物素部分数量的方法。该测定法包括在添加生物素化蛋白前后滴定链霉抗生物素蛋白/抗生物素蛋白上生物素结合位点的数量。使用这种方法，仅能检测到那些能够与链霉抗生物素蛋白/抗生物素蛋白结合的生物素部分。该测定法简单且灵敏，每次测定仅需1 - 10微克生物素化蛋白。
