Yu Sa, Zhang Jun, Moran Marina S, Lu Jun Q, Feng Yuanming, Hu Xin-Hua
Department of Biomedical Engineering, Tianjin University, Tianjin 300072, China.
Opt Express. 2012 Sep 24;20(20):22245-51. doi: 10.1364/OE.20.022245.
We report a novel method of diffraction imaging flow cytometry to measure and analyze size distribution of microspheres. An automated and robust image processing software based on the short-time-Fourier-transform algorithm has been developed to analyze the characteristic and spatially varying oscillations of side scatters recorded as a diffraction image. Our results demonstrate that the new method allows accurate and rapid determination of single microspheres' diameters ranging from 1 to 100 μm. The capacity for analysis of light scattering by two-sphere aggregates has been demonstrated but analytical tools for characterization of aggregates by multiple microspheres remain to be developed.
我们报告了一种用于测量和分析微球尺寸分布的新型衍射成像流式细胞术方法。已开发出一种基于短时傅里叶变换算法的自动化且稳健的图像处理软件，用于分析记录为衍射图像的侧向散射光的特征和空间变化振荡。我们的结果表明，该新方法能够准确、快速地测定直径范围为1至100μm的单个微球。已证明该方法具有分析双球聚集体光散射的能力，但用于表征多个微球聚集体的分析工具仍有待开发。
