Khmaladze Alexander, Kim Myung, Lo Chun-Min
Department of Physics, University of South Florida, Tampa, FL 33620, USA.
Opt Express. 2008 Jul 21;16(15):10900-11. doi: 10.1364/oe.16.010900.
We present a phase-imaging technique to quantitatively study the three-dimensional structure of cells. The method, based on the simultaneous dual-wavelength digital holography, allows for higher axial range at which the unambiguous phase imaging can be performed. The technique is capable of nanometer axial resolution. The noise level, which increases as a result of using two wavelengths, is then reduced to the level of a single wavelength. The method compares favorably to software unwrapping, as the technique does not produce non-existent phase steps. Curvature mismatch between the reference and object beams is numerically compensated. The 3D images of SKOV-3 ovarian cancer cells are presented.
我们提出一种相位成像技术，用于定量研究细胞的三维结构。该方法基于同时双波长数字全息术，可实现更高的轴向范围，在此范围内能够进行明确的相位成像。该技术具备纳米级轴向分辨率。由于使用两个波长而增加的噪声水平随后被降低到单波长水平。该方法与软件解包裹相比具有优势，因为该技术不会产生不存在的相位步长。参考光束和物光束之间的曲率失配通过数值方法进行补偿。展示了SKOV-3卵巢癌细胞的三维图像。
