酶调控的未修饰金纳米颗粒聚集：一种无标记比色法测定腺苷脱氨酶活性和抑制的快速灵敏检测方法。
Enzyme-regulated unmodified gold nanoparticle aggregation: a label free colorimetric assay for rapid and sensitive detection of adenosine deaminase activity and inhibition.
机构信息
State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha 410082, P. R. China.
出版信息
Chem Commun (Camb). 2012 Nov 18;48(89):10996-8. doi: 10.1039/c2cc36240f.
PMID:23037591
Abstract
Based on enzyme-regulated unmodified gold nanoparticle aggregation, a visual and homogeneous assay of adenosine deaminase (ADA) activity without any other coupling enzymes, aptamers or additional modifications has been developed. The present strategy is simple, cost-effective, high throughput, selective and sensitive for ADA with a detection limit of 0.8227 U L(-1).
摘要
基于酶调控的未修饰金纳米颗粒聚集，开发了一种无需任何其他偶联酶、适体或额外修饰的腺苷脱氨酶（ADA）活性的可视化均相测定法。本策略具有简单、经济高效、高通量、选择性和灵敏度高的特点，ADA 的检测限为 0.8227 U L(-1)。
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