Aro H T, Wippermann B W, Hodgson S F, Chao E Y
Department of Orthopedics, Mayo Clinic/Mayo Foundation, Rochester, MN 55905.
J Orthop Res. 1990 Mar;8(2):238-46. doi: 10.1002/jor.1100080213.
A closed fracture model of the rat tibia was employed to study internal remodeling of periosteal new bone during fracture repair. Static histomorphometric parameters of osteoid surface (or perimeter) and eroded surface (resorption surface) were used as indicators of appositional bone formation and resorption of bone trabeculae, respectively. Intracortical remodeling at the fracture site was evaluated using quantitative tetracycline histology and microradiography. The extents of osteoid and eroded bone surfaces did not differ significantly in the periosteal woven new bone in the early phases of fracture healing. Later on, the periosteal new bone had significantly more osteoid surface than eroded surface (p less than 0.001). The number of osteoclasts also decreased significantly over time during fracture healing (p = 0.028). Cortical bone showed a continuous increase of porosity (p less than 0.01) between 1 and 6 weeks after fracture. These results suggest that there is a time-related change in the balance of periosteal bone formation and resorption during the progress of fracture repair. We hypothesize that this change was related to the restoration of bony continuity. Further studies are, however, needed to indicate the histomorphometric features of periosteal new bone in fracture nonunions.
采用大鼠胫骨闭合性骨折模型来研究骨折修复过程中骨膜新生骨的内部重塑。类骨质表面（或周长）和侵蚀表面（吸收表面）的静态组织形态计量学参数分别用作骨小梁贴壁骨形成和吸收的指标。使用定量四环素组织学和显微放射ography评估骨折部位的皮质内重塑。在骨折愈合早期，骨膜编织状新生骨中的类骨质和侵蚀骨表面范围无显著差异。后来，骨膜新生骨的类骨质表面明显多于侵蚀表面（p小于0.001）。在骨折愈合过程中，破骨细胞数量也随时间显著减少（p = 0.028）。骨折后1至6周期间，皮质骨孔隙率持续增加（p小于0.01）。这些结果表明，在骨折修复过程中，骨膜骨形成和吸收的平衡存在与时间相关的变化。我们假设这种变化与骨连续性的恢复有关。然而，需要进一步研究来表明骨折不愈合中骨膜新生骨的组织形态计量学特征。
