Wang Lingjun, Mao Jiannan, Cai Feng, Tang Jincheng, Xi Kun, Feng Yu, Xu Yichang, Liang Xiao, Gu Yong, Chen Liang
Department of Orthopaedics, The First Affiliated Hospital of Soochow University, Suzhou, China.
Department of Orthopaedics, The Affiliated Jiangyin Hospital of Nantong University Medical College, Jiang Yin, China.
Front Bioeng Biotechnol. 2022 Jul 22;10:927050. doi: 10.3389/fbioe.2022.927050. eCollection 2022.
The regeneration of critical-size bone defects on long bones has remained a significant challenge because of the complex anatomical structure and vascular network. In such circumstances, current biomaterial forms with homogeneous structure and function can hardly satisfy the need for both osteogenesis and angiogenesis. In the current study, a heterogeneous biomimetic structured scaffold was constructed with the help of a 3D printed mold to simultaneously mimic the outer/inner periosteum and intermediate bone matrix of a natural long bone. Because of the reinforcement modified mesoporous bioactive glass nanoparticles (MBGNs), enhanced structural stability and adequate osteogenic capacity could be achieved for the intermediate layer of this scaffold. Conversely, GelMA incorporated with VEGF-loaded liposome exhibiting controlled release of the angiogenic factor was applied to the inner and outer layers of the scaffold. The resulting heterogeneous structured scaffold was shown to successfully guide bone regeneration and restoration of the natural bone anatomic structure, rendering it a promising candidate for future orthopedic clinical studies.
由于长骨复杂的解剖结构和血管网络，临界尺寸骨缺损的再生仍然是一项重大挑战。在这种情况下，目前具有均匀结构和功能的生物材料形式很难满足骨生成和血管生成的需求。在本研究中，借助3D打印模具构建了一种异质仿生结构支架，以同时模拟天然长骨的外层/内层骨膜和中间骨基质。由于增强改性的介孔生物活性玻璃纳米颗粒（MBGNs），该支架的中间层可实现增强的结构稳定性和足够的成骨能力。相反，掺入载有VEGF的脂质体并表现出血管生成因子可控释放的GelMA被应用于支架的内层和外层。结果表明，所得的异质结构支架成功地引导了骨再生并恢复了天然骨的解剖结构，使其成为未来骨科临床研究的有希望的候选者。
