Lee Ho-Sung, Lee Byung-Cheon
Pharmacopuncture Medical Research Center, Korean Pharmacopuncture Institute, Seoul, South Korea.
J Acupunct Meridian Stud. 2012 Oct;5(5):218-25. doi: 10.1016/j.jams.2012.07.005. Epub 2012 Aug 9.
By spraying and injecting Alcian blue into the lateral ventricle, we were able to visualize the network of the nerve primo vascular system above the pia mater of the brain and spine of rats. Staining these novel structures above the pia mater with 4',6-diamidino-2-phenylindole demonstrated that they coexisted in cellular and extracellular DNA forms. The cellular primo node consisted of many cells surrounded by rod-shaped nuclei while the extracellular primo node had a different morphology from that of a general cell in terms of DNA signals, showing granular DNA in a threadlike network of extracellular DNA. Also, differently from F-actin in general cells, the F-actin in the primo vessel was short and rod-shaped. Light and transmission electron microscopic images of the PN showed that the nerve primo vascular system above the pia mater of the brain and spine was a novel dynamic network, suggesting the coexistence of DNA and extracellular DNA. Based on these data, we suggest that a novel dynamic system with a certain function exists above the pia mater of the central nerve system. We also discuss the potential of this novel network system in the brain and spine as related to acupuncture meridians and neural regeneration.
通过向大鼠侧脑室喷洒和注射阿尔新蓝，我们能够观察到大鼠脑和脊髓软脑膜上方的神经始祖血管系统网络。用4',6-二脒基-2-苯基吲哚对软脑膜上方的这些新结构进行染色，结果表明它们以细胞内和细胞外DNA形式共存。细胞始祖结由许多被杆状核包围的细胞组成，而细胞外始祖结在DNA信号方面具有与普通细胞不同的形态，在细胞外DNA的丝状网络中显示出颗粒状DNA。此外，与普通细胞中的F-肌动蛋白不同，始祖血管中的F-肌动蛋白短且呈杆状。始祖结的光学和透射电子显微镜图像显示，脑和脊髓软脑膜上方的神经始祖血管系统是一个新的动态网络，表明DNA和细胞外DNA共存。基于这些数据，我们认为在中枢神经系统软脑膜上方存在一个具有一定功能的新动态系统。我们还讨论了这个新网络系统在脑和脊髓中与针灸经络及神经再生相关的潜力。
