Lee Ho-Sung, Kang Dai-In, Yoon Seung Zhoo, Ryu Yeon Hee, Lee Inhyung, Kim Hoon-Gi, Lee Byung-Cheon, Lee Ki Bog
Ki Primo Research Laboratory, Division of Electrical Engineering, KAIST Institute for Information Technology Convergence, Korea Advanced Institute of Science and Technology (KAIST), Daejeon, Korea ; Nadi Primo Research Institute, Graduate School of Integrative Medicine, Sun Moon University, Asan-si, Korea.
Pharmacopuncture Medical Research Center, Korean Pharmacopuncture Institute, Seoul, Korea.
Neural Regen Res. 2015 Jul;10(7):1101-6. doi: 10.4103/1673-5374.160103.
With chromium-hematoxylin staining, we found evidence for the existence of novel age-dependent network structures in the dura mater of rat brains. Under stereomicroscopy, we noticed that chromium-hematoxylin-stained threadlike structures, which were barely observable in 1-week-old rats, were networked in specific areas of the brain, for example, the lateral lobes and the cerebella, in 4-week-old rats. In 7-week-old rats, those structures were found to have become larger and better networked. With phase contrast microscopy, we found that in 1-week-old rats, chromium-hematoxylin-stained granules were scattered in the same areas of the brain in which the network structures would later be observed in the 4- and 7-week-old rats. Such age-dependent network structures were examined by using optical and transmission electron microscopy, and the following results were obtained. The scattered granules fused into networks with increasing age. Cross-sections of the age-dependent network structures demonstrated heavily-stained basophilic substructures. Transmission electron microscopy revealed the basophilic substructures to be clusters with high electron densities consisting of nanosized particles. We report these data as evidence for the existence of age-dependent network structures in the dura mater, we discuss their putative functions of age-dependent network structures beyond the general concept of the dura mater as a supporting matrix.
通过铬苏木精染色，我们发现大鼠硬脑膜中存在新的年龄依赖性网络结构的证据。在体视显微镜下，我们注意到在1周龄大鼠中几乎观察不到的铬苏木精染色的丝状结构，在4周龄大鼠的大脑特定区域，如外侧叶和小脑，形成了网络。在7周龄大鼠中，这些结构变得更大且网络更完善。通过相差显微镜，我们发现，在1周龄大鼠中，铬苏木精染色的颗粒散在于大脑中与4周龄和7周龄大鼠后来观察到网络结构相同的区域。使用光学显微镜和透射电子显微镜对这种年龄依赖性网络结构进行了检查，得到了以下结果。随着年龄增长，分散的颗粒融合成网络。年龄依赖性网络结构的横截面显示出嗜碱性强的亚结构。透射电子显微镜显示嗜碱性亚结构是由纳米级颗粒组成的高电子密度簇。我们报告这些数据作为硬脑膜中存在年龄依赖性网络结构的证据，我们讨论了它们除了硬脑膜作为支持基质的一般概念之外的年龄依赖性网络结构的假定功能。
