Stem Cell Unit, Department of Genetics, Institute of Life Sciences, The Hebrew University, Jerusalem 91904, Israel.
Curr Opin Genet Dev. 2012 Oct;22(5):444-9. doi: 10.1016/j.gde.2012.09.003. Epub 2012 Oct 4.
The genetic stability of induced pluripotent stem (iPS) cells has a significant impact on their potential use in regenerative medicine and basic research. Analysis of the genomic integrity of iPS cells suggests a tendency to develop aberrations ranging from whole chromosome trisomies to single nucleotide mutations. Furthermore, fluctuations in telomere elongation and changes in mitochondrial DNA are also observed. Some mutations may already exist in the founder cells or result from prolonged culturing, however, many of the mutations occur during the reprogramming event. Thus, great care should be given to the initial characterization and subsequent culturing of new iPS cell lines in order to avoid the use of potentially aberrant cells.
诱导多能干细胞（iPS 细胞）的遗传稳定性对其在再生医学和基础研究中的潜在应用有重大影响。对 iPS 细胞基因组完整性的分析表明，存在从整条染色体三体到单个核苷酸突变等各种异常的趋势。此外，还观察到端粒延长的波动和线粒体 DNA 的变化。一些突变可能已经存在于起始细胞中，或者是由于长期培养引起的，但许多突变是在重编程过程中发生的。因此，在对新的 iPS 细胞系进行初始特征描述和后续培养时，应格外小心，以避免使用可能存在异常的细胞。
