针对明尼苏达沙门氏菌R595脂多糖产生的多克隆抗体和单克隆抗体的特异性及生物活性比较。
A comparison of specificity and biological activity of polyclonal and monoclonal antibodies raised against Salmonella minnesota R595 lipopolysaccharide.
作者信息
Mehta N D, Wilson B M, Rapson N T, Easmon C S
机构信息
Department of Hematology/Oncology, Michael Reese Hospital and Medical Center, Chicago, IL.
出版信息
J Med Microbiol. 1990 Feb;31(2):85-93. doi: 10.1099/00222615-31-2-85.
Murine monoclonal antibodies (MAbs) and immune rabbit serum were raised against the rough mutant of Salmonella minnesota strain R595. These antibodies were tested for their ability to inhibit LPS-induced B-cell mitogenicity and neutralise LPS toxicity in chick embryos. Immune rabbit serum inhibited both mitogenicity and LPS lethality. None of the MAbs or a cocktail of antibodies were able to neutralise LPS lethality in chick embryos. However, they were able to inhibit mitogenicity by varying degrees.
制备了针对明尼苏达沙门氏菌R595粗糙突变株的鼠单克隆抗体（MAb）和免疫兔血清。测试了这些抗体抑制脂多糖（LPS）诱导的B细胞有丝分裂活性以及中和LPS对鸡胚毒性的能力。免疫兔血清抑制了有丝分裂活性和LPS致死性。单克隆抗体或抗体混合物均不能中和LPS对鸡胚的致死性。然而，它们能够不同程度地抑制有丝分裂活性。
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