Boston University, Department of Biomedical Engineering, Boston, Massachusetts 02215, USA.
Opt Lett. 2012 Sep 15;37(18):3798-800. doi: 10.1364/ol.37.003798.
We introduce a single-exposure widefield system of producing phase gradient images by dividing the illumination and detection apertures into two oblique complementary components and encoding them using polarization. A Wollaston prism splits the images formed by the two respective aperture halves to allow both components to be simultaneously imaged by a single camera. By producing images characteristically similar to differential interference contrast while using a darkfield illumination scheme, sensitivity to weak phase gradients is improved.
我们介绍了一种单曝光宽场系统，通过将照明和探测孔径分成两个斜向互补的部分，并使用偏振进行编码来产生相位梯度图像。渥拉斯顿棱镜将由两个孔径半部分形成的图像分开，以使单个相机能够同时对两个部分进行成像。通过使用暗场照明方案产生类似于微分干涉对比的图像，提高了对弱相位梯度的灵敏度。
