Boston University, Department of Biomedical Engineering, Boston, Massachusetts 02215, USA.
J Biomed Opt. 2013 Jun;18(6):066007. doi: 10.1117/1.JBO.18.6.066007.
Oblique back-illumination microscopy (OBM) is a new technique for simultaneous, independent measurements of phase gradients and absorption in thick scattering tissues based on widefield imaging. To date, OBM has been used with sequential camera exposures, which reduces temporal resolution, and can produce motion artifacts in dynamic samples. Here, a variation of OBM that allows single-exposure operation with wavelength multiplexing and image splitting with a Wollaston prism is introduced. Asymmetric anamorphic distortion induced by the prism is characterized and corrected in real time using a graphics-processing unit. To demonstrate the capacity of single-exposure OBM to perform artifact-free imaging of blood flow, video-rate movies of microcirculation in ovo in the chorioallantoic membrane of the developing chick are presented. Imaging is performed with a high-resolution rigid Hopkins lens suitable for endoscopy.
斜向背反射显微镜（OBM）是一种新的技术，可基于宽场成像对厚散射组织中的相位梯度和吸收进行同时、独立的测量。迄今为止，OBM 已与逐次相机曝光一起使用，这降低了时间分辨率，并且在动态样本中可能产生运动伪影。此处，介绍了一种允许单曝光操作、波长复用和渥拉斯顿棱镜分光的 OBM 变体。使用图形处理单元实时对棱镜引起的非对称变形进行特征描述和校正。为了证明单曝光 OBM 具有无伪影成像血流的能力，展示了发育中的鸡胚尿囊绒毛膜中小循环的视频速率电影。使用适合内窥镜的高分辨率刚性 Hopkins 透镜进行成像。
