Department of Biomedical Engineering, University of Wisconsin-Madison, Madison, Wisconsin 53706, USA.
Opt Lett. 2012 Sep 15;37(18):3837-9. doi: 10.1364/ol.37.003837.
We report the use of second-harmonic generation (SHG) microscopy in conjunction with circular dichroism (CD) to differentiate normal skin from that in the connective tissue disorder osteogenesis imperfecta (OI). Osteogenesis imperfecta results from mutations in the collagen triple helix, where the individual chains are defective, leading to abnormal folding, and ultimately, abnormal fibril/fiber organization. Second-harmonic-generation circular dichroism successfully differentiated normal human and OI skin tissues, whereas other SHG polarization schemes did not provide discrimination, suggesting this approach has high sensitivity for studying the difference in chirality in the mutated collagen. We further suggest that the method has clinical diagnostic value, as it could be performed with minimal invasion.
我们报告了使用二次谐波产生（SHG）显微镜结合圆二色性（CD）来区分正常皮肤和结缔组织疾病成骨不全症（OI）的皮肤。成骨不全症是由于胶原蛋白三螺旋中的突变，导致单个链缺陷，从而导致异常折叠，并最终导致异常原纤维/纤维组织。二次谐波产生圆二色性成功地区分了正常人和 OI 皮肤组织，而其他 SHG 偏振方案则没有提供区分，这表明这种方法对研究突变胶原蛋白手性差异具有很高的灵敏度。我们进一步建议该方法具有临床诊断价值，因为它可以进行最小侵入性操作。
