Beijing Institute of Radiation Medicine, State Key Laboratory of Proteomics, State Key Laboratory of Medical Neurobiology, Cognitive and Mental Health Research Center, Beijing, China.
J Histochem Cytochem. 2013 Jan;61(1):45-54. doi: 10.1369/0022155412461255. Epub 2012 Oct 5.
The present study aimed to evaluate the expression of neuro-oncological ventral antigen 1 (Nova1) in cerebral ischemia/reperfusion (I/R) insults by immunohistochemistry. The focal cerebral I/R model was induced by right middle cerebral artery occlusion (MCAO) for 120 min followed by 1 day, 7 days, and 14 days of reperfusion in Sprague-Dawley (SD) rats. The results showed that Nova1 was expressed in nearly the whole brain, although with higher density in hippocampus, hypothalamus, cingulate cortex, and medial habenular nucleus. The immunoreactivity of Nova1 neurons was increased dramatically, especially on both sides of the hippocampal CA(1) region, after 1 day of reperfusion. A strong response occurred at the ipsilateral CA(1) region between 1 day and 7 days of reperfusion. Likewise, strong compensatory responses of Nova1 expression were observed on the contralateral side of the striate cortex, dentate gyrus, and hypothalamus. Interestingly, more Nova1 neurons were observed to translocate to the dendrites and growth cones of the axons in the hypothalamus on the ischemic side after 7 days of reperfusion. In conclusion, our data suggest that Nova1 might mediate neuronal responsiveness, and its expression might positively correlate with neural repair after I/R insults in the rat brain.
本研究旨在通过免疫组织化学方法评估神经肿瘤学腹侧抗原 1（Nova1）在脑缺血/再灌注（I/R）损伤中的表达。采用右侧大脑中动脉闭塞（MCAO）法诱导 Sprague-Dawley（SD）大鼠局灶性脑 I/R 模型，再灌注 1 天、7 天和 14 天。结果表明，Nova1 在全脑中均有表达，但在海马、下丘脑、扣带回和内侧缰核中表达密度更高。Nova1 神经元的免疫反应性在再灌注 1 天后显著增强，尤其是在海马 CA（1）区的两侧。在再灌注 1 天至 7 天期间，同侧 CA（1）区出现强烈反应。同样，纹状体皮层、齿状回和下丘脑对侧也观察到 Nova1 表达的强烈代偿反应。有趣的是，再灌注 7 天后，缺血侧下丘脑的 Nova1 神经元更多地迁移到轴突的树突和生长锥中。总之，我们的数据表明，Nova1 可能介导神经元反应性，其表达可能与大鼠脑 I/R 损伤后的神经修复呈正相关。
