Kudo T, Kudo M, Muraoka M, Amano N, Matsuki A
Department of Anesthesiology, University of Hirosaki School of Medicine.
Masui. 1990 Jan;39(1):81-4.
An accurate and simplified method has been developed for determination of ketamine in human plasma using gas chromatography and electron impact mode mass spectrometry with selected ion recording from 0.5ml of plasma. Standards and samples of plasma underwent the same procedure of two step extraction by methanol. Ketamine concentrations in the plasma were determined from the peak in the selected ion profile of ketamine (m/e: 237). Standard curve was linear with the increasing amount of ketamine (0.63-5.0 micrograms.ml-1) in plasma with mean CV = 5.2% mean RR = 63.8% and r = 0.998. The concentration of ketamine in plasma ranged from 1.6 micrograms.ml-1 to 2.5 micrograms.ml-1 during ketamine anesthesia (2mg.kg-1.h-1) in a surgical patient.
已开发出一种准确且简化的方法，用于使用气相色谱法和电子轰击模式质谱法，通过选择离子记录，从0.5毫升血浆中测定人体血浆中的氯胺酮。血浆标准品和样品均经过相同的甲醇两步萃取程序。根据氯胺酮选择离子图谱中的峰（m/e：237）测定血浆中的氯胺酮浓度。血浆中氯胺酮含量增加时，标准曲线呈线性（0.63 - 5.0微克·毫升⁻¹），平均变异系数（CV）= 5.2%，平均相对回收率（RR）= 63.8%，r = 0.998。在一名外科手术患者接受氯胺酮麻醉（2毫克·千克⁻¹·小时⁻¹）期间，血浆中氯胺酮浓度范围为1.6微克·毫升⁻¹至2.5微克·毫升⁻¹。
