Keilwerth Melanie, Bühler Ilina, Hoffmann Rudolf, Soliman Hatem, El-Matbouli Mansour
Klinik für Fische und Reptilien, Tierärztliche Fakultät der Ludwig-Maximilians-Universität München, Deutschland.
Berl Munch Tierarztl Wochenschr. 2012 Sep-Oct;125(9-10):411-7.
Our objective was to evaluate diagnostic tools for the detection of Inclusion Body Disease (IBD) in bold snakes. The aetiology of IBD is unknown, and the disease has non-specific clinical signs, hence there is a need for a clinically-applicable, specific diagnostic method. We examined blood smears and liver biopsies from 26 bold snakes (17 boas and nine pythons; some of which were suspected of having IBD) for the presence of characteristic inclusion bodies. We used haematology, histology and electron microscopy to characterise samples as IBD-positive or -negative. Our results indicate that examination of a simple blood smear is sufficient to diagnose IBD in boas. Inclusion bodies in lymphocytes, erythrocytes and thrombocytes were observed. In both, boas and pythons, we detected inclusion bodies within hepatocytes. We demonstrated also that IBD was more common in boas than in pythons: only samples from two Ball Pythons (Python regius) tested positive, whereas no other Pythonidae were positive. We consider that blood smears represents a rapid, non-invasive technique for detection of IBD.
我们的目标是评估用于检测巨蛇包涵体病（IBD）的诊断工具。IBD的病因尚不清楚，且该病具有非特异性临床症状，因此需要一种临床适用的特异性诊断方法。我们检查了26条巨蛇（17条蟒和9条蚺；其中一些怀疑患有IBD）的血涂片和肝活检组织，以查找特征性包涵体的存在。我们使用血液学、组织学和电子显微镜将样本鉴定为IBD阳性或阴性。我们的结果表明，检查简单的血涂片就足以诊断蟒类的IBD。在淋巴细胞、红细胞和血小板中观察到了包涵体。在蟒和蚺中，我们都在肝细胞内检测到了包涵体。我们还证明，IBD在蟒类中比在蚺类中更常见：仅来自两条球蟒（球蟒属）的样本检测呈阳性，而其他蚺科动物均为阴性。我们认为血涂片是检测IBD的一种快速、非侵入性技术。
