细胞色素c过氧化物酶过氧化氢化合物的电子-核双共振：用甲硫氨酸簇鉴定自由基位点。
Electron-nuclear double resonance of the hydrogen peroxide compound of cytochrome c peroxidase: identification of the free radical site with a methionyl cluster.
作者信息
Hoffman B M, Roberts J E, Brown T G, Kang C H, Margoliash E
出版信息
Proc Natl Acad Sci U S A. 1979 Dec;76(12):6132-6. doi: 10.1073/pnas.76.12.6132.
Abstract
The results of electron-nuclear double resonance and electron paramagnetic resonance (EPR) studies on the hydrogen peroxide compound of yeast cytochrome c peroxidase are inconsistent with previous proposals for the source of the EPR signal in this compound, in particular with its identification with an aromatic amino acid radical such as would arise by oxidation of a tryptophanyl side chain. The present observations lead us to propose that the EPR signal is associated with a cluster containing at least one methionine and in which proximate side chains share the charge created by loss of one electron.
摘要
对酵母细胞色素c过氧化物酶的过氧化氢化合物进行的电子-核双共振和电子顺磁共振（EPR）研究结果与该化合物中EPR信号来源的先前提议不一致，特别是与将其鉴定为芳香族氨基酸自由基（如色氨酸侧链氧化产生的自由基）不一致。目前的观察结果使我们提出，EPR信号与一个至少包含一个甲硫氨酸的簇相关，并且邻近的侧链共享因失去一个电子而产生的电荷。
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本文引用的文献
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