• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

有证据表明禽肉瘤病毒转化基因产物是一种不依赖环磷酸腺苷的蛋白激酶。

Evidence that the avian sarcoma virus transforming gene product is a cyclic AMP-independent protein kinase.

作者信息

Erikson R L, Collett M S, Erikson E, Purchio A F

出版信息

Proc Natl Acad Sci U S A. 1979 Dec;76(12):6260-4. doi: 10.1073/pnas.76.12.6260.

DOI:10.1073/pnas.76.12.6260
PMID:230504
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC411843/
Abstract

The avian sarcoma virus transforming gene product pp60src has been partially purified by using ion exchange or immunoaffinity chromatography. These preparations contain a cyclic AMP-independent protein kinase activity capable of transferring radiolabel from [gamma-32P]ATP to immune rabbit IgG, casein, and the heavy chain purified from normal rabbit IgG. The casein kinase activity is specifically inhibited by anti-pp60src IgG. Comparison of the pp60src-protein kinase isolated from cells transformed by a wild-type ASV or by an ASV temperature-sensitive transformation mutant revealed that the latter product had a half-life of 3 min at 41 degrees C whereas that of the wild-type product was 20 min.

摘要

禽肉瘤病毒转化基因产物pp60src已通过离子交换或免疫亲和层析进行了部分纯化。这些制剂含有一种不依赖环磷酸腺苷的蛋白激酶活性，能够将放射性标记从[γ-32P]ATP转移至免疫兔IgG、酪蛋白以及从正常兔IgG中纯化的重链。酪蛋白激酶活性受到抗pp60src IgG的特异性抑制。对从野生型ASV或ASV温度敏感转化突变体转化的细胞中分离出的pp60src蛋白激酶进行比较发现，后一种产物在41℃时的半衰期为3分钟，而野生型产物的半衰期为20分钟。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/baeb/411843/b22ba9fd3780/pnas00012-0247-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/baeb/411843/32140232b047/pnas00012-0245-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/baeb/411843/08386d52839c/pnas00012-0245-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/baeb/411843/3716e42a8f19/pnas00012-0245-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/baeb/411843/aada35708a26/pnas00012-0246-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/baeb/411843/e0d098d8a974/pnas00012-0246-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/baeb/411843/b22ba9fd3780/pnas00012-0247-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/baeb/411843/32140232b047/pnas00012-0245-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/baeb/411843/08386d52839c/pnas00012-0245-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/baeb/411843/3716e42a8f19/pnas00012-0245-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/baeb/411843/aada35708a26/pnas00012-0246-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/baeb/411843/e0d098d8a974/pnas00012-0246-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/baeb/411843/b22ba9fd3780/pnas00012-0247-a.jpg

相似文献

1
Evidence that the avian sarcoma virus transforming gene product is a cyclic AMP-independent protein kinase.有证据表明禽肉瘤病毒转化基因产物是一种不依赖环磷酸腺苷的蛋白激酶。
Proc Natl Acad Sci U S A. 1979 Dec;76(12):6260-4. doi: 10.1073/pnas.76.12.6260.
2
Avian sarcoma virus-transforming protein, pp60src shows protein kinase activity specific for tyrosine.禽肉瘤病毒转化蛋白pp60src表现出对酪氨酸具有特异性的蛋白激酶活性。
Nature. 1980 May 15;285(5761):167-9. doi: 10.1038/285167a0.
3
Molecular events in cells transformed by Rous Sarcoma virus.劳氏肉瘤病毒转化细胞中的分子事件。
J Cell Biol. 1980 Nov;87(2 Pt 1):319-25. doi: 10.1083/jcb.87.2.319.
4
Src Gene product from different strains of avian sarcoma virus: Kinetics and possible mechanism of heat inactivation of protein kinase activity from cells infected by transformation-defective, temperature-sensitive mutant and wild-type virus.来自不同禽肉瘤病毒株的Src基因产物：转化缺陷型、温度敏感型突变体和野生型病毒感染细胞中蛋白激酶活性热失活的动力学及可能机制。
Proc Natl Acad Sci U S A. 1979 Feb;76(2):967-71. doi: 10.1073/pnas.76.2.967.
5
Transforming gene product of avian sarcoma viruses and its homolog in uninfected cells.禽肉瘤病毒的转化基因产物及其在未感染细胞中的同源物。
Prog Clin Biol Res. 1980;41:747-56.
6
The src gene product of transformed and morphologically reverted ASV-infected mammalian cells.转化的和形态学上回复的禽肉瘤病毒感染的哺乳动物细胞的src基因产物。
Nature. 1979 Sep 20;281(5728):195-8. doi: 10.1038/281195a0.
7
Towards a molecular description of cell transformation by avian sarcoma virus.迈向对禽肉瘤病毒引起细胞转化的分子描述。
Proc R Soc Lond B Biol Sci. 1980 Nov 19;210(1180):387-96. doi: 10.1098/rspb.1980.0141.
8
Studies on the structure and function of the avian sarcoma virus transforming-gene product.禽肉瘤病毒转化基因产物的结构与功能研究。
Cold Spring Harb Symp Quant Biol. 1980;44 Pt 2,:931-41. doi: 10.1101/sqb.1980.044.01.100.
9
Protein kinase activity associated with the avian sarcoma virus src gene product.与禽肉瘤病毒src基因产物相关的蛋白激酶活性。
Proc Natl Acad Sci U S A. 1978 Apr;75(4):2021-4. doi: 10.1073/pnas.75.4.2021.
10
Loss of tumorigenicity correlates with a reduction in pp60src kinase activity in a revertant subclone of avian sarcoma virus-infected field vole cells.在禽肉瘤病毒感染的田鼠细胞的一个回复性子克隆中，致瘤性的丧失与pp60src激酶活性的降低相关。
Cell. 1981 Mar;23(3):815-23. doi: 10.1016/0092-8674(81)90446-3.

引用本文的文献

1
Tyrosine-protein kinase Yes controls endothelial junctional plasticity and barrier integrity by regulating VE-cadherin phosphorylation and endocytosis.酪氨酸蛋白激酶Yes通过调节血管内皮钙黏蛋白的磷酸化和内吞作用来控制内皮细胞连接可塑性和屏障完整性。
Nat Cardiovasc Res. 2022 Dec 7;1(12):1156-1173. doi: 10.1038/s44161-022-00172-z.
2
Biographical Feature: Marc S. Collett (23 May 1951-11 June 2022): the Battle against Viral Disease Has Lost a Valiant Warrior, and the World Has Lost a Splendid Human Being.生平特写：马克·S·科利特（1951年5月23日 - 2022年6月11日）：抗击病毒性疾病的战斗失去了一位英勇的战士，世界失去了一位杰出的人物。
J Virol. 2023 Jan 31;97(1):e0164322. doi: 10.1128/jvi.01643-22. Epub 2022 Dec 5.
3

本文引用的文献

1
A film detection method for tritium-labelled proteins and nucleic acids in polyacrylamide gels.一种用于检测聚丙烯酰胺凝胶中氚标记蛋白质和核酸的胶片检测方法。
Eur J Biochem. 1974 Jul 1;46(1):83-8. doi: 10.1111/j.1432-1033.1974.tb03599.x.
2
The effects of reciprocal changes in temperature on the transformed state of cells infected with a rous sarcoma virus mutant.温度的相互变化对感染劳氏肉瘤病毒突变体的细胞转化状态的影响。
Virology. 1971 Nov;46(2):470-9. doi: 10.1016/0042-6822(71)90047-x.
3
Rapid isolation of antigens from cells with a staphylococcal protein A-antibody adsorbent: parameters of the interaction of antibody-antigen complexes with protein A.
Dynamic regulatory features of the protein tyrosine kinases.
蛋白质酪氨酸激酶的动态调控特征。
Biochem Soc Trans. 2019 Aug 30;47(4):1101-1116. doi: 10.1042/BST20180590. Epub 2019 Aug 8.
4
Identification of phosphotyrosine-containing proteins in untransformed and Rous sarcoma virus-transformed chicken embryo fibroblasts.未转化和劳斯肉瘤病毒转化的鸡胚成纤维细胞中含磷酸酪氨酸蛋白的鉴定。
Mol Cell Biol. 1982 Jun;2(6):653-65. doi: 10.1128/mcb.2.6.653-665.1982.
5
Synthetic peptide fragment of src gene product inhibits the src protein kinase and crossreacts immunologically with avian onc kinases and cellular phosphoproteins.src基因产物的合成肽片段可抑制src蛋白激酶，并与禽肿瘤激酶和细胞磷蛋白发生免疫交叉反应。
Proc Natl Acad Sci U S A. 1981 Dec;78(12):7412-6. doi: 10.1073/pnas.78.12.7412.
6
Only site-directed antibodies reactive with the highly conserved src-homologous region of the v-abl protein neutralize kinase activity.只有与v-abl蛋白高度保守的src同源区域发生反应的位点特异性抗体才能中和激酶活性。
J Virol. 1984 Jul;51(1):223-32. doi: 10.1128/JVI.51.1.223-232.1984.
7
Tyrosine phosphorylation of specific proteins after mitogen stimulation of chicken embryo fibroblasts.丝裂原刺激鸡胚成纤维细胞后特定蛋白质的酪氨酸磷酸化
Mol Cell Biol. 1983 Mar;3(3):380-90. doi: 10.1128/mcb.3.3.380-390.1983.
8
Highly purified pp60src induces the actin transformation in microinjected cells and phosphorylates selected cytoskeletal proteins in vitro.高度纯化的pp60src在显微注射的细胞中诱导肌动蛋白转化，并在体外使选定的细胞骨架蛋白磷酸化。
Mol Cell Biol. 1983 Jan;3(1):102-12. doi: 10.1128/mcb.3.1.102-112.1983.
9
The p36 substrate of tyrosine-specific protein kinases co-localizes with non-erythrocyte alpha-spectrin antigen, p230, in surface lamina of cultured fibroblasts.酪氨酸特异性蛋白激酶的p36底物与非红细胞α-血影蛋白抗原p230在培养成纤维细胞的表面板中共定位。
EMBO J. 1983;2(10):1701-5. doi: 10.1002/j.1460-2075.1983.tb01645.x.
10
Subcellular location of an abundant substrate (p36) for tyrosine-specific protein kinases.酪氨酸特异性蛋白激酶的一种丰富底物（p36）的亚细胞定位。
Mol Cell Biol. 1983 Mar;3(3):340-50. doi: 10.1128/mcb.3.3.340-350.1983.
用葡萄球菌蛋白A-抗体吸附剂从细胞中快速分离抗原：抗体-抗原复合物与蛋白A相互作用的参数
J Immunol. 1975 Dec;115(6):1617-24.
4
Product of in vitro translation of the Rous sarcoma virus src gene has protein kinase activity.劳氏肉瘤病毒src基因的体外翻译产物具有蛋白激酶活性。
J Virol. 1979 Apr;30(1):311-8. doi: 10.1128/JVI.30.1.311-318.1979.
5
Structural analysis of the avian sarcoma virus transforming protein: sites of phosphorylation.禽肉瘤病毒转化蛋白的结构分析：磷酸化位点
J Virol. 1979 Feb;29(2):770-81. doi: 10.1128/JVI.29.2.770-781.1979.
6
Src Gene product from different strains of avian sarcoma virus: Kinetics and possible mechanism of heat inactivation of protein kinase activity from cells infected by transformation-defective, temperature-sensitive mutant and wild-type virus.来自不同禽肉瘤病毒株的Src基因产物：转化缺陷型、温度敏感型突变体和野生型病毒感染细胞中蛋白激酶活性热失活的动力学及可能机制。
Proc Natl Acad Sci U S A. 1979 Feb;76(2):967-71. doi: 10.1073/pnas.76.2.967.
7
Evidence that the transforming gene of avian sarcoma virus encodes a protein kinase associated with a phosphoprotein.禽肉瘤病毒的转化基因编码一种与磷蛋白相关的蛋白激酶的证据。
Cell. 1978 Oct;15(2):561-72. doi: 10.1016/0092-8674(78)90024-7.
8
In vitro synthesis of a functional avian sarcoma virus transforming-gene product.功能性禽肉瘤病毒转化基因产物的体外合成
Nature. 1978 Aug 31;274(5674):919-21. doi: 10.1038/274919a0.
9
Peptide analysis of the transformation-specific antigen from avian sarcoma virus-transformed cells.禽肉瘤病毒转化细胞中转化特异性抗原的肽分析。
J Virol. 1978 Jun;26(3):773-82. doi: 10.1128/JVI.26.3.773-782.1978.
10
Identification of a polypeptide encoded by the avian sarcoma virus src gene.禽肉瘤病毒src基因编码的一种多肽的鉴定。
Proc Natl Acad Sci U S A. 1978 Mar;75(3):1567-71. doi: 10.1073/pnas.75.3.1567.