Tan Yanjun, Wang Hengjuan, Song Yan, Yang Hui, Jia Xudong, Li Ning
National Institute for Nutrition and Food Safety, China CDC, Beijing 100021, China.
Wei Sheng Yan Jiu. 2012 May;41(3):399-404.
To study the potential endocrine disrupting effects of bifenthrin (BIF) by using uterotrophic assay and Hershberger assay.
In uterotrophic assay, 60 female SD rats were randomly divided into 6 groups, 10 rats per group. Rats in bifenthrin-treated groups were given different doses of bifenthrin (1.47, 4.41 and 13.23 mg/kg BW by gavage for 3 consecutive days). Rats in negative control groups were given corn oil by gavage. Rats in ethinyl estradiol (EE) oral positive control groups were given EE 1.0 microg/kg BW by gavage. Rats in EE injected positive groups were given 0.6 microg/kg BW EE by subcutaneously injection while given corn oil by gavage. At necropsy, the wet and blotted uteri were weighed. The relative uteri weights were calculated, and the histology of uteri was observed. In Hershberger assay, 60 castrated male SD rats were randomly divided into 6 groups, with 10 rats in each group. Rats in BIF-treated groups were given different doses of BIF (1.4, 4.2 and 12.6 mg/kg BW) by gavage. Flutamide (3.0 mg/kg BW) were given to animals in the positive control group by gavage. Rats in the negative control group and testosterone propionate group were given corn oil by gavage for 10 consecutive days. Rats in all groups except the negative control group were also treated with testosterone propionate (TP, 0.2 mg/kg BW) by subcutaneous injection. At necropsy, ventral prostate (VP), seminal vesicle plus fluids and coagulating glands (SVCG), levator ani-bulbocavernosus muscle (LABC), paired Cowper's glands (COW) and the glands penis (GP), liver, kidneys, adrenals were weighed. Serum triiodothyronine (T3) and thyroxine (T4) were determined.
In uterotrophic assay, compared with the negative control group, the mean relative wet weight and relative blotted weight of uterine were increased significantly in the female rats given by BIF at 13.23 mg/kg BW for 3 days (P < 0.05). BIF resulted in a significant increase of epithelial cell heights of uteri at 4.41 and 13.23 mg/kg BW, as compared with control group. In Hershberger assay, compared with the TP group, mean absolute weight and relative weight of VP and LABC in BIF high dose group were significant decreased. Moreover, total T3 and T4 level were decreased also (P < 0.05).
Uterotrophic assay and Hershberger assay used in this study showed that BIF may have endocrine disrupting properties.
通过子宫增重试验和赫什伯格试验研究联苯菊酯（BIF）潜在的内分泌干扰作用。
在子宫增重试验中，将60只雌性SD大鼠随机分为6组，每组10只。联苯菊酯处理组的大鼠连续3天经口灌胃给予不同剂量的联苯菊酯（1.47、4.41和13.23mg/kg体重）。阴性对照组的大鼠经口灌胃给予玉米油。乙炔雌二醇（EE）口服阳性对照组的大鼠经口灌胃给予1.0μg/kg体重的EE。EE注射阳性组的大鼠皮下注射给予0.6μg/kg体重的EE，同时经口灌胃给予玉米油。剖检时，称量子宫的湿重和吸干后的重量。计算子宫相对重量，并观察子宫组织学变化。在赫什伯格试验中，将60只去势雄性SD大鼠随机分为6组，每组10只。联苯菊酯处理组的大鼠经口灌胃给予不同剂量的联苯菊酯（1.4、4.2和12.6mg/kg体重）。阳性对照组的动物经口灌胃给予氟他胺（3.0mg/kg体重）。阴性对照组和丙酸睾酮组的大鼠连续10天经口灌胃给予玉米油。除阴性对照组外，所有组的大鼠还皮下注射给予丙酸睾酮（TP，0.2mg/kg体重）。剖检时，称量腹侧前列腺（VP）、精囊加精囊液和凝固腺（SVCG）、提肛肌-球海绵体肌（LABC）、成对的考珀氏腺（COW）和阴茎腺（GP）、肝脏、肾脏、肾上腺的重量。测定血清三碘甲状腺原氨酸（T3）和甲状腺素（T4）。
在子宫增重试验中，与阴性对照组相比，连续3天给予13.23mg/kg体重联苯菊酯的雌性大鼠子宫的平均相对湿重和相对吸干重显著增加（P<0.05）。与对照组相比，联苯菊酯在4.41和13.23mg/kg体重时导致子宫上皮细胞高度显著增加。在赫什伯格试验中，与TP组相比，联苯菊酯高剂量组VP和LABC的平均绝对重量和相对重量显著降低。此外，总T3和T4水平也降低（P<0.05）。
本研究中使用的子宫增重试验和赫什伯格试验表明，联苯菊酯可能具有内分泌干扰特性。
