Agroprocessing and Natural Products Division, Council of Scientific and Industrial Research-National Institute for Interdisciplinary Science and Technology, Thiruvananthapuram, Kerala, India.
J Cardiovasc Pharmacol. 2013 Jan;61(1):23-34. doi: 10.1097/FJC.0b013e3182756ad3.
Cardiac hypertrophy occurs in response to increased workload, such as hypertension or valvular heart disease. Oxidative stress has been implicated in cardiac hypertrophy and in its transition to heart failure. This study was taken up with the objective to evaluate the role of oxidative stress in cardiomyoblast hypertrophy and its modulation by Desmodium gangeticum (DG) that has been traditionally used in Ayurveda, an Indian system of medicine. The methanolic root extract was analyzed for total phenolic content and tested for antioxidant potential. Hypertrophy was induced by exposing H9c2 cell line to β-adrenergic receptor agonist, isoproterenol (ISO), for 96 hours. Analyses of reactive oxygen species (ROS) generation, mitochondrial transmembrane potential ([INCREMENT]Ψm), and integrity of permeability transition were performed in ISO as well as Desmodium and ISO-cotreated cells. The results demonstrated potent free radical scavenging activity of DG. Cell line studies showed significant increase in ROS generation, dissipation of [INCREMENT]Ψm, and permeability transition pore opening in ISO-treated cells. Desmodium was found to attenuate ISO-induced hypertrophy by reduction of ROS generation, restoration of [INCREMENT]Ψm, and prevention of permeability transition pore opening. This study is the first documentation of the modulatory effect of DG on cardiac hypertrophy.
心肌肥厚是对增加的工作量（如高血压或心脏瓣膜病）的反应。氧化应激已被牵涉到心肌肥厚及其向心力衰竭的转变中。本研究旨在评估氧化应激在心肌细胞肥大中的作用，以及传统上用于阿育吠陀医学（印度医学体系）的 Desmodium gangeticum（DG）对其的调节作用。对 DG 的甲醇根提取物进行总酚含量分析，并测试其抗氧化潜力。通过用β-肾上腺素能受体激动剂异丙肾上腺素（ISO）处理 H9c2 细胞系 96 小时来诱导肥大。在 ISO 以及 Desmodium 和 ISO 共同处理的细胞中进行活性氧（ROS）生成、线粒体跨膜电位（[INCREMENT]Ψm）和通透性转换孔完整性的分析。结果表明 DG 具有很强的清除自由基活性。细胞系研究表明，ISO 处理的细胞中 ROS 生成显著增加，[INCREMENT]Ψm 耗散和通透性转换孔开放。发现 Desmodium 通过减少 ROS 生成、恢复[INCREMENT]Ψm 和防止通透性转换孔开放来减轻 ISO 诱导的肥大。这项研究首次记录了 DG 对心肌肥厚的调节作用。
