用于检测儿童上呼吸道感染细菌病原体的替代采样方法。
Alternative sampling methods for detecting bacterial pathogens in children with upper respiratory tract infections.
机构信息
Department of Pediatric Immunology and Infectious Diseases, University Medical Center Utrecht, Wilhelmina Children's Hospital, Utrecht, the Netherlands.
出版信息
J Clin Microbiol. 2012 Dec;50(12):4134-7. doi: 10.1128/JCM.02376-12. Epub 2012 Oct 10.
Abstract
Nasopharyngeal sampling is used for detecting bacteria commonly involved in upper respiratory tract infections, but it requires training and may not always be well tolerated. We sampled children (n = 66) of ages 0 to 4 years, with rhinorrhea, by using a nasopharyngeal swab, a nasal swab, and nose blowing/wiping into a paper tissue. Streptococcus pneumoniae, Haemophilus influenzae, Moraxella catarrhalis, and Staphylococcus aureus were cultured at similar rates across methods with high concordance (80 to 97%), indicating that they are reliably detected by alternative means.
摘要
鼻咽取样常用于检测上呼吸道感染常见的细菌，但需要经过培训，而且并非总能被患者接受。我们用鼻咽拭子、鼻腔拭子和向纸巾擤鼻/擦拭的方式对 0 至 4 岁有流涕症状的儿童（n = 66）进行了取样。肺炎链球菌、流感嗜血杆菌、卡他莫拉菌和金黄色葡萄球菌在各种方法中的培养率相似，具有很高的一致性（80%至 97%），表明它们可以通过替代方法可靠地检测到。
相似文献
1
Alternative sampling methods for detecting bacterial pathogens in children with upper respiratory tract infections.用于检测儿童上呼吸道感染细菌病原体的替代采样方法。
J Clin Microbiol. 2012 Dec;50(12):4134-7. doi: 10.1128/JCM.02376-12. Epub 2012 Oct 10.
2
Nasopharyngeal versus oropharyngeal sampling for isolation of potential respiratory pathogens in adults.成人鼻咽与口咽采样用于分离潜在呼吸道病原体的研究
J Clin Microbiol. 2006 Feb;44(2):525-8. doi: 10.1128/JCM.44.2.525-528.2006.
3
Evaluation of swabbing methods for estimating the prevalence of bacterial carriage in the upper respiratory tract: a cross sectional study.评估用于估计上呼吸道细菌携带率的擦拭方法：一项横断面研究。
BMJ Open. 2014 Oct 30;4(10):e005341. doi: 10.1136/bmjopen-2014-005341.
4
Comparison between nasal swabs and nasopharyngeal aspirates for, and effect of time in transit on, isolation of Streptococcus pneumoniae, Staphylococcus aureus, Haemophilus influenzae, and Moraxella catarrhalis.鼻拭子与鼻咽抽吸物在肺炎链球菌、金黄色葡萄球菌、流感嗜血杆菌和卡他莫拉菌分离方面的比较以及转运时间对其的影响
J Clin Microbiol. 2007 Jan;45(1):244-5. doi: 10.1128/JCM.01131-06. Epub 2006 Nov 1.
5
Clinical characteristics of children with lower respiratory tract infections are dependent on the carriage of specific pathogens in the nasopharynx.儿童下呼吸道感染的临床特征取决于鼻咽部特定病原体的携带情况。
Eur J Clin Microbiol Infect Dis. 2012 Nov;31(11):3173-82. doi: 10.1007/s10096-012-1682-y. Epub 2012 Aug 1.
6
The prevalence of potentially pathogenic bacteria in nasopharyngeal samples from individuals with a long-standing cough-clinical value of a nasopharyngeal sample.长期咳嗽患者鼻咽样本中潜在致病菌的患病率——鼻咽样本的临床价值
Fam Pract. 2000 Apr;17(2):150-5. doi: 10.1093/fampra/17.2.150.
7
Pathogenic bacterial nasopharyngeal colonization and its impact on respiratory diseases in the first year of life: the PATCH Birth Cohort Study.致病性细菌在鼻咽部的定植及其对生命第一年呼吸道疾病的影响：PATCH出生队列研究
Pediatr Infect Dis J. 2015 Jun;34(6):652-8. doi: 10.1097/INF.0000000000000688.
8
Airway bacterial carriage and childhood respiratory health: A population-based prospective cohort study.气道细菌携带与儿童呼吸道健康：基于人群的前瞻性队列研究。
Pediatr Allergy Immunol. 2020 Oct;31(7):774-782. doi: 10.1111/pai.13310. Epub 2020 Jun 30.
9
Quantification of nasopharyngeal bacteria for diagnosis of respiratory tract infection in children.
Scand J Infect Dis. 1990;22(3):333-7. doi: 10.3109/00365549009027056.
10
Nasopharyngeal polymicrobial colonization during health, viral upper respiratory infection and upper respiratory bacterial infection.健康状态、病毒性上呼吸道感染及上呼吸道细菌感染期间的鼻咽部多种微生物定植
J Infect. 2017 Jul;75(1):26-34. doi: 10.1016/j.jinf.2017.04.003. Epub 2017 Apr 13.
引用本文的文献
1
Across-Shift Changes in Viable Nasal Bacteria among Waste-Incineration Plant Workers-A Pilot Study.跨班次变化对垃圾焚烧厂工人鼻腔内可培养细菌的影响：一项初步研究。
Int J Environ Res Public Health. 2022 Jul 23;19(15):8984. doi: 10.3390/ijerph19158984.
2
The characterization of carried in the general population.一般人群中携带的特征。
Microb Genom. 2022 May;8(5). doi: 10.1099/mgen.0.000820.
3
Nasopharyngeal Swabs vs. Nasal Aspirates for Respiratory Virus Detection: A Systematic Review.鼻咽拭子与鼻腔吸取物用于呼吸道病毒检测的系统评价
Pathogens. 2021 Nov 20;10(11):1515. doi: 10.3390/pathogens10111515.
4
Tracheal colonization factor A (TcfA) is a biomarker for rapid and specific detection of Bordetella pertussis.气管定植因子A（TcfA）是一种用于快速、特异性检测百日咳博德特氏菌的生物标志物。
Sci Rep. 2020 Sep 14;10(1):15002. doi: 10.1038/s41598-020-72092-6.
5
Nasal swab bacteriology by PCR during the first 24-months of life: A prospective birth cohort study.生后 24 个月内鼻拭子聚合酶链反应细菌学：一项前瞻性出生队列研究。
Pediatr Pulmonol. 2019 Mar;54(3):289-296. doi: 10.1002/ppul.24231. Epub 2019 Jan 4.
6
Ribosomal PCR assay of excised intervertebral discs from patients undergoing single-level primary lumbar microdiscectomy.对接受单节段原发性腰椎间盘显微切除术患者切除的椎间盘进行核糖体聚合酶链反应检测。
Eur Spine J. 2017 Aug;26(8):2038-2044. doi: 10.1007/s00586-017-5141-4. Epub 2017 May 31.
7
Detection of Streptococcus pneumoniae from Different Types of Nasopharyngeal Swabs in Children.儿童不同类型鼻咽拭子中肺炎链球菌的检测
PLoS One. 2013 Jun 26;8(6):e68097. doi: 10.1371/journal.pone.0068097. Print 2013.
本文引用的文献
1
The potter's wheel: the host's role in sculpting its microbiota.陶轮：塑造其微生物群的主人角色。
Cell Mol Life Sci. 2011 Nov;68(22):3675-85. doi: 10.1007/s00018-011-0830-3. Epub 2011 Oct 4.
2
Immunogenicity, safety, and reactogenicity of the 10-valent pneumococcal nontypeable Haemophilus influenzae protein D conjugate vaccine and DTPa-IPV-Hib when coadministered as a 3-dose primary vaccination schedule in The Netherlands: a randomized controlled trial.免疫原性、安全性和反应原性的 10 价肺炎球菌结合疫苗和 DTPa-IPV-Hib 无血清型流感嗜血杆菌蛋白 D 当共同作为一个 3 剂量主要免疫接种程序在荷兰:一项随机对照试验。
Pediatr Infect Dis J. 2011 Sep;30(9):e170-8. doi: 10.1097/INF.0b013e31821a0614.
3
Moraxella catarrhalis, a human respiratory tract pathogen.卡他莫拉菌，一种人类呼吸道病原体。
Clin Infect Dis. 2009 Jul 1;49(1):124-31. doi: 10.1086/599375.
4
Comparison of nasal swabs with nose blowing for community-based pneumococcal surveillance of healthy children.鼻拭子与擤鼻用于健康儿童社区肺炎球菌监测的比较
J Clin Microbiol. 2008 Jun;46(6):2081-2. doi: 10.1128/JCM.00048-08. Epub 2008 Apr 2.
5
Influence of sampling technique on detection of potential pathogens in the nasopharynx.
Arch Otolaryngol Head Neck Surg. 2006 Jul;132(7):752-5. doi: 10.1001/archotol.132.7.752.
6
Evaluation of the Quidel QuickVue test for detection of influenza A and B viruses in the pediatric emergency medicine setting by use of three specimen collection methods.通过三种标本采集方法评估Quidel QuickVue检测法在儿科急诊医学环境中检测甲型和乙型流感病毒的效果。
J Clin Microbiol. 2006 Jul;44(7):2638-41. doi: 10.1128/JCM.02644-05.
7
Relative importance of nasopharyngeal versus oropharyngeal sampling for isolation of Streptococcus pneumoniae and Haemophilus influenzae from healthy and sick individuals varies with age.从健康个体和患病个体中分离肺炎链球菌和流感嗜血杆菌时，鼻咽采样与口咽采样的相对重要性因年龄而异。
J Clin Microbiol. 2004 Oct;42(10):4604-9. doi: 10.1128/JCM.42.10.4604-4609.2004.
8
Effects of rhinovirus infection on the adherence of Streptococcus pneumoniae to cultured human airway epithelial cells.鼻病毒感染对肺炎链球菌黏附于培养的人气道上皮细胞的影响。
J Infect Dis. 2003 Dec 15;188(12):1928-39. doi: 10.1086/379833. Epub 2003 Dec 3.
9
Healthy carriage of Staphylococcus aureus: its prevalence and importance.金黄色葡萄球菌的健康携带状态：其流行情况及重要性。
Bacteriol Rev. 1963 Mar;27(1):56-71. doi: 10.1128/br.27.1.56-71.1963.
10
Report from a WHO Working Group: standard method for detecting upper respiratory carriage of Streptococcus pneumoniae.世界卫生组织工作组报告：检测肺炎链球菌上呼吸道携带情况的标准方法
Pediatr Infect Dis J. 2003 Feb;22(2):e1-11. doi: 10.1097/01.inf.0000049347.42983.77.
Zotero 插件