用于检测儿童上呼吸道感染细菌病原体的替代采样方法。
Alternative sampling methods for detecting bacterial pathogens in children with upper respiratory tract infections.
机构信息
Department of Pediatric Immunology and Infectious Diseases, University Medical Center Utrecht, Wilhelmina Children's Hospital, Utrecht, the Netherlands.
出版信息
J Clin Microbiol. 2012 Dec;50(12):4134-7. doi: 10.1128/JCM.02376-12. Epub 2012 Oct 10.
Abstract
Nasopharyngeal sampling is used for detecting bacteria commonly involved in upper respiratory tract infections, but it requires training and may not always be well tolerated. We sampled children (n = 66) of ages 0 to 4 years, with rhinorrhea, by using a nasopharyngeal swab, a nasal swab, and nose blowing/wiping into a paper tissue. Streptococcus pneumoniae, Haemophilus influenzae, Moraxella catarrhalis, and Staphylococcus aureus were cultured at similar rates across methods with high concordance (80 to 97%), indicating that they are reliably detected by alternative means.
摘要
鼻咽取样常用于检测上呼吸道感染常见的细菌，但需要经过培训，而且并非总能被患者接受。我们用鼻咽拭子、鼻腔拭子和向纸巾擤鼻/擦拭的方式对 0 至 4 岁有流涕症状的儿童（n = 66）进行了取样。肺炎链球菌、流感嗜血杆菌、卡他莫拉菌和金黄色葡萄球菌在各种方法中的培养率相似，具有很高的一致性（80%至 97%），表明它们可以通过替代方法可靠地检测到。
相似文献
1
Alternative sampling methods for detecting bacterial pathogens in children with upper respiratory tract infections.
J Clin Microbiol. 2012 Dec;50(12):4134-7. doi: 10.1128/JCM.02376-12. Epub 2012 Oct 10.
2
Nasopharyngeal versus oropharyngeal sampling for isolation of potential respiratory pathogens in adults.
J Clin Microbiol. 2006 Feb;44(2):525-8. doi: 10.1128/JCM.44.2.525-528.2006.
3
Evaluation of swabbing methods for estimating the prevalence of bacterial carriage in the upper respiratory tract: a cross sectional study.
BMJ Open. 2014 Oct 30;4(10):e005341. doi: 10.1136/bmjopen-2014-005341.
4
Comparison between nasal swabs and nasopharyngeal aspirates for, and effect of time in transit on, isolation of Streptococcus pneumoniae, Staphylococcus aureus, Haemophilus influenzae, and Moraxella catarrhalis.
J Clin Microbiol. 2007 Jan;45(1):244-5. doi: 10.1128/JCM.01131-06. Epub 2006 Nov 1.
5
Clinical characteristics of children with lower respiratory tract infections are dependent on the carriage of specific pathogens in the nasopharynx.
Eur J Clin Microbiol Infect Dis. 2012 Nov;31(11):3173-82. doi: 10.1007/s10096-012-1682-y. Epub 2012 Aug 1.
6
The prevalence of potentially pathogenic bacteria in nasopharyngeal samples from individuals with a long-standing cough-clinical value of a nasopharyngeal sample.
Fam Pract. 2000 Apr;17(2):150-5. doi: 10.1093/fampra/17.2.150.
7
Pathogenic bacterial nasopharyngeal colonization and its impact on respiratory diseases in the first year of life: the PATCH Birth Cohort Study.
Pediatr Infect Dis J. 2015 Jun;34(6):652-8. doi: 10.1097/INF.0000000000000688.
8
Airway bacterial carriage and childhood respiratory health: A population-based prospective cohort study.
Pediatr Allergy Immunol. 2020 Oct;31(7):774-782. doi: 10.1111/pai.13310. Epub 2020 Jun 30.
9
Quantification of nasopharyngeal bacteria for diagnosis of respiratory tract infection in children.
Scand J Infect Dis. 1990;22(3):333-7. doi: 10.3109/00365549009027056.
10
Nasopharyngeal polymicrobial colonization during health, viral upper respiratory infection and upper respiratory bacterial infection.
J Infect. 2017 Jul;75(1):26-34. doi: 10.1016/j.jinf.2017.04.003. Epub 2017 Apr 13.
引用本文的文献
1
Across-Shift Changes in Viable Nasal Bacteria among Waste-Incineration Plant Workers-A Pilot Study.
Int J Environ Res Public Health. 2022 Jul 23;19(15):8984. doi: 10.3390/ijerph19158984.
2
The characterization of carried in the general population.
Microb Genom. 2022 May;8(5). doi: 10.1099/mgen.0.000820.
3
Nasopharyngeal Swabs vs. Nasal Aspirates for Respiratory Virus Detection: A Systematic Review.
Pathogens. 2021 Nov 20;10(11):1515. doi: 10.3390/pathogens10111515.
4
Tracheal colonization factor A (TcfA) is a biomarker for rapid and specific detection of Bordetella pertussis.
Sci Rep. 2020 Sep 14;10(1):15002. doi: 10.1038/s41598-020-72092-6.
5
Nasal swab bacteriology by PCR during the first 24-months of life: A prospective birth cohort study.
Pediatr Pulmonol. 2019 Mar;54(3):289-296. doi: 10.1002/ppul.24231. Epub 2019 Jan 4.
6
Ribosomal PCR assay of excised intervertebral discs from patients undergoing single-level primary lumbar microdiscectomy.
Eur Spine J. 2017 Aug;26(8):2038-2044. doi: 10.1007/s00586-017-5141-4. Epub 2017 May 31.
7
Detection of Streptococcus pneumoniae from Different Types of Nasopharyngeal Swabs in Children.
PLoS One. 2013 Jun 26;8(6):e68097. doi: 10.1371/journal.pone.0068097. Print 2013.
本文引用的文献
1
The potter's wheel: the host's role in sculpting its microbiota.
Cell Mol Life Sci. 2011 Nov;68(22):3675-85. doi: 10.1007/s00018-011-0830-3. Epub 2011 Oct 4.
2
Immunogenicity, safety, and reactogenicity of the 10-valent pneumococcal nontypeable Haemophilus influenzae protein D conjugate vaccine and DTPa-IPV-Hib when coadministered as a 3-dose primary vaccination schedule in The Netherlands: a randomized controlled trial.
Pediatr Infect Dis J. 2011 Sep;30(9):e170-8. doi: 10.1097/INF.0b013e31821a0614.
3
Moraxella catarrhalis, a human respiratory tract pathogen.
Clin Infect Dis. 2009 Jul 1;49(1):124-31. doi: 10.1086/599375.
4
Comparison of nasal swabs with nose blowing for community-based pneumococcal surveillance of healthy children.
J Clin Microbiol. 2008 Jun;46(6):2081-2. doi: 10.1128/JCM.00048-08. Epub 2008 Apr 2.
5
Influence of sampling technique on detection of potential pathogens in the nasopharynx.
Arch Otolaryngol Head Neck Surg. 2006 Jul;132(7):752-5. doi: 10.1001/archotol.132.7.752.
6
Evaluation of the Quidel QuickVue test for detection of influenza A and B viruses in the pediatric emergency medicine setting by use of three specimen collection methods.
J Clin Microbiol. 2006 Jul;44(7):2638-41. doi: 10.1128/JCM.02644-05.
7
Relative importance of nasopharyngeal versus oropharyngeal sampling for isolation of Streptococcus pneumoniae and Haemophilus influenzae from healthy and sick individuals varies with age.
J Clin Microbiol. 2004 Oct;42(10):4604-9. doi: 10.1128/JCM.42.10.4604-4609.2004.
8
Effects of rhinovirus infection on the adherence of Streptococcus pneumoniae to cultured human airway epithelial cells.
J Infect Dis. 2003 Dec 15;188(12):1928-39. doi: 10.1086/379833. Epub 2003 Dec 3.
9
Healthy carriage of Staphylococcus aureus: its prevalence and importance.
Bacteriol Rev. 1963 Mar;27(1):56-71. doi: 10.1128/br.27.1.56-71.1963.
10
Report from a WHO Working Group: standard method for detecting upper respiratory carriage of Streptococcus pneumoniae.
Pediatr Infect Dis J. 2003 Feb;22(2):e1-11. doi: 10.1097/01.inf.0000049347.42983.77.
Zotero 插件